[3dem] Interpretation of strange objects in a cryo-EM sample

[vincent Chaptal]

Dear James,

you didn't say which detergent the micelles would be orinigating from, 
it changes from detergent to detergent.

But in any case, beside the mix DDM/CHS which forms small micelles when 
concentrated but large objects when diluted, most micelles are much much 
smaller, say 30-50Å or so.

I think there is an article by the group of Arne Moeller on detergent 
micelle observation by negative stain, I don't recall if they tried in 
cryo.

Best
Vincent

Le 13/04/2023 à 15:48, Kizziah, James a écrit :
>
> Hi all,
>
> We have imaged a cryo-EM sample presumed to contain micelles and 
> observed the large, white objects full of/covered with junk shown in 
> the attached images. Our best guess is that these are (or were) the 
> micelles and have been destroyed by the electron beam, contact with 
> the air water interface, or both, after vitrification. Has anyone seen 
> something like this before or does anyone have alternative 
> interpretations?
>
> Glow-discharged lacey carbon grids plunge frozen with a Mark IV 
> Vitrobot, clipped and imaged on a Glacios.
>
> Thanks,
>
> James
>
>
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-- 

Vincent Chaptal, PhD

Director of GdR APPICOM

Drug Resistance and Membrane Proteins Lab


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