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<font size="4"><font face="monospace">Dear James, <br>
<br>
you didn't say which detergent the micelles would be orinigating
from, it changes from detergent to detergent. <br>
<br>
But in any case, beside the mix DDM/CHS which forms small
micelles when concentrated but large objects when diluted, most
micelles are much much smaller, say 30-50Å or so. <br>
<br>
I think there is an article by the group of Arne Moeller on
detergent micelle observation by negative stain, I don't recall
if they tried in cryo. <br>
<br>
Best<br>
Vincent<br>
</font></font><br>
<div class="moz-cite-prefix">Le 13/04/2023 à 15:48, Kizziah, James a
écrit :<br>
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<p class="MsoNormal"
style="line-height:120%;text-autospace:none">Hi all,<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none"><o:p> </o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">We have imaged
a cryo-EM sample presumed to contain micelles and observed
the large, white objects full of/covered with junk shown in
the attached images. Our best guess is that these are (or
were) the micelles and have been destroyed by the electron
beam, contact with the air water interface, or both, after
vitrification. Has anyone seen something like this before or
does anyone have alternative interpretations?
<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none"><o:p> </o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">Glow-discharged
lacey carbon grids plunge frozen with a Mark IV Vitrobot,
clipped and imaged on a Glacios.
<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none"><o:p> </o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">Thanks,<o:p></o:p></p>
<p class="MsoNormal"
style="line-height:120%;text-autospace:none">James<o:p></o:p></p>
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<br>
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<pre class="moz-quote-pre" wrap="">_______________________________________________
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<p class="p1"><span class="s1">Vincent Chaptal, PhD</span></p>
<p class="p1"><span class="s1">Director of GdR APPICOM</span></p>
<p class="p1"><span class="s1">Drug Resistance and Membrane
Proteins Lab</span></p>
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<p class="p1"><span class="s1">FRANCE</span></p>
<p class="p1"><span class="s1">+33 4 37 65 29 01</span></p>
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