[3dem] unknown structures

[Matthias Wolf]

Hi Roman,

The first thing I thought of is viral mini-genomes like the RNA in your MS2 paper.
I have seen viruses stripped off their capsids like that before - clustered RNA has high contrast, in particular at 200 kV or lower.

You could run a gel and stain for nucleic acid, but I guess you already know more... ;-)

Best regards,
   Matthias

From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of r.i.koning at lumc.nl
Sent: Monday, October 10, 2022 9:46 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] unknown structures

Dear all,

I would like to have you expert opinion on what the structures in the attached image might be. The structures are quite dense in my opinion. I have my ideas but I am not at all sure. I hope any of you have seen similar structures.

Best regards,

Roman

Dr. R.I.Koning

Leiden University Medical Center, Cell and Chemical Biology, Electron Microscopy
Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands

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