[3dem] unknown structures

[r.i.koning at lumc.nl]

Dear all,

Thank you all for your input and suggestions (via my direct e-mail). Very helpful.

Most people suggested some kind of Ca containing (CaP) aggregates, similar to inclusions that can be found in mitochondria and which apparently can be formed in PBS buffers where there also is Ca present.

Literature research led me to two articles with very similar structures which indeed were Ca containing aggregates (Xu, Y., Tijssen, K.C.H., Bomans, P.H.H. et al. Microscopic structure of the polymer-induced liquid precursor for calcium carbonate. Nat Commun 9, 2582 (2018). https://urldefense.com/v3/__https://doi.org/10.1038/s41467-018-05006-w__;!!Mih3wA!FpjoTJKGoBdFoDMndgcQWhAKCoLpgCDvMLi2XlowXaZJXhKyfB8bgJAv1xQuksASY_IYoprwv6veylySXhKdoKU$  ; Habraken, W., Tao, J., Brylka, L. et al. Ion-association complexes unite classical and non-classical theories for the biomimetic nucleation of calcium phosphate. Nat Commun 4, 1507 (2013). https://urldefense.com/v3/__https://doi.org/10.1038/ncomms2490__;!!Mih3wA!FpjoTJKGoBdFoDMndgcQWhAKCoLpgCDvMLi2XlowXaZJXhKyfB8bgJAv1xQuksASY_IYoprwv6veylySDvkDYyY$  ).

I will check the buffers and beam sensitivity of the samples (and possibly EDX/EELS) to check all this.

Best regards,

Roman


Dr. R.I.Koning

Leiden University Medical Center, Cell and Chemical Biology, Electron Microscopy
Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands

visiting address. Einthovenweg 20, Building 2, zone S1-P, room R-90-21
tel. (31) 71 526 9296 fax. (+31) 71 526 8270 mail. r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl>

From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of r.i.koning at lumc.nl
Sent: maandag 10 oktober 2022 14:46
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] unknown structures

Dear all,

I would like to have you expert opinion on what the structures in the attached image might be. The structures are quite dense in my opinion. I have my ideas but I am not at all sure. I hope any of you have seen similar structures.

Best regards,

Roman

Dr. R.I.Koning

Leiden University Medical Center, Cell and Chemical Biology, Electron Microscopy
Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands

visiting address. Einthovenweg 20, Building 2, zone S1-P, room R-90-21
tel. (31) 71 526 9296 fax. (+31) 71 526 8270 mail. r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl> web. https://urldefense.com/v3/__http://www.kosterlab.nl__;!!Mih3wA!FpjoTJKGoBdFoDMndgcQWhAKCoLpgCDvMLi2XlowXaZJXhKyfB8bgJAv1xQuksASY_IYoprwv6veylyS6zFtv4w$  <https://urldefense.com/v3/__http:/www.kosterlab.nl/__;!!Mih3wA!F4lNir_quXjQA0OCOnJTcn8SALwmpJlIYa7sS_mNNlUXvFr8spA-OUWkVBosGs3LAI6wkv09XnIncfYrM5h64LI$>


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