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<p class="MsoNormal"><span lang="EN-US">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Thank you all for your input and suggestions (via my direct e-mail). Very helpful.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Most people suggested some kind of Ca containing (CaP) aggregates, similar to inclusions that can be found in mitochondria and which apparently can be formed in PBS buffers where there also is Ca present.
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Literature research led me to two articles with very similar structures which indeed were Ca containing aggregates (<span style="color:#222222;background:white">Xu, Y., Tijssen, K.C.H., Bomans, P.H.H. <i>et al.</i> Microscopic
structure of the polymer-induced liquid precursor for calcium carbonate. </span></span><i><span style="color:#222222;background:white">Nat Commun</span></i><span style="color:#222222;background:white"> <b>9</b>, 2582 (2018). <a href="https://urldefense.com/v3/__https://doi.org/10.1038/s41467-018-05006-w__;!!Mih3wA!FpjoTJKGoBdFoDMndgcQWhAKCoLpgCDvMLi2XlowXaZJXhKyfB8bgJAv1xQuksASY_IYoprwv6veylySXhKdoKU$">https://doi.org/10.1038/s41467-018-05006-w</a>;
Habraken, W., Tao, J., Brylka, L. et al. </span><span lang="EN-US" style="color:#222222;background:white">Ion-association complexes unite classical and non-classical theories for the biomimetic nucleation of calcium phosphate. Nat Commun 4, 1507 (2013). <a href="https://urldefense.com/v3/__https://doi.org/10.1038/ncomms2490__;!!Mih3wA!FpjoTJKGoBdFoDMndgcQWhAKCoLpgCDvMLi2XlowXaZJXhKyfB8bgJAv1xQuksASY_IYoprwv6veylySDvkDYyY$">https://doi.org/10.1038/ncomms2490</a></span><span lang="EN-US">).<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">I will check the buffers and beam sensitivity of the samples (and possibly EDX/EELS) to check all this.
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Best regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Roman<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal" align="center" style="text-align:center"><b><span lang="EN-US" style="color:gray;mso-fareast-language:NL">Dr. R.I.Koning<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="color:gray;mso-fareast-language:NL"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-GB" style="color:gray;mso-fareast-language:NL">Leiden University Medical Center, Cell and Chemical Biology, Electron Microsc</span><span lang="EN-US" style="color:gray;mso-fareast-language:NL">opy<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="color:gray;mso-fareast-language:NL">Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="color:gray;mso-fareast-language:NL"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span lang="EN-US" style="color:gray;mso-fareast-language:NL">visiting address.</span></b><span lang="EN-US" style="color:gray;mso-fareast-language:NL"> Einthovenweg 20, Building 2, zone S1-P,
room R-90-21<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span lang="EN-US" style="color:gray;mso-fareast-language:NL">tel.</span></b><span lang="EN-US" style="color:gray;mso-fareast-language:NL"> (31) 71 526 9296
<b>fax.</b> (+31) 71 526 8270 <b>mail. </b></span><span lang="EN-US" style="mso-fareast-language:NL"><a href="mailto:r.i.koning@lumc.nl"><span style="color:blue">r.i.koning@lumc.nl</span></a><span style="color:gray">
<o:p></o:p></span></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="mso-fareast-language:NL">From:</span></b><span lang="EN-US" style="mso-fareast-language:NL"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>r.i.koning@lumc.nl<br>
<b>Sent:</b> maandag 10 oktober 2022 14:46<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] unknown structures<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">I would like to have you expert opinion on what the structures in the attached image might be. The structures are quite dense in my opinion. I have my ideas but I am not at all sure. I hope any of you have seen similar
structures. <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Best regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Roman <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span lang="EN-US" style="color:gray;mso-fareast-language:NL">Dr. R.I.Koning<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="color:gray;mso-fareast-language:NL"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-GB" style="color:gray;mso-fareast-language:NL">Leiden University Medical Center, Cell and Chemical Biology, Electron Microsc</span><span lang="EN-US" style="color:gray;mso-fareast-language:NL">opy<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="color:gray;mso-fareast-language:NL">Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="color:gray;mso-fareast-language:NL"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span lang="EN-US" style="color:gray;mso-fareast-language:NL">visiting address.</span></b><span lang="EN-US" style="color:gray;mso-fareast-language:NL"> Einthovenweg 20, Building 2, zone S1-P,
room R-90-21<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span lang="EN-US" style="color:gray;mso-fareast-language:NL">tel.</span></b><span lang="EN-US" style="color:gray;mso-fareast-language:NL"> (31) 71 526 9296
<b>fax.</b> (+31) 71 526 8270 <b>mail. </b></span><span lang="EN-US" style="mso-fareast-language:NL"><a href="mailto:r.i.koning@lumc.nl"><span style="color:blue">r.i.koning@lumc.nl</span></a><span style="color:gray">
<b>web.</b> </span><a href="https://urldefense.com/v3/__http:/www.kosterlab.nl/__;!!Mih3wA!F4lNir_quXjQA0OCOnJTcn8SALwmpJlIYa7sS_mNNlUXvFr8spA-OUWkVBosGs3LAI6wkv09XnIncfYrM5h64LI$"><span style="color:blue">www.kosterlab.nl</span></a><span style="color:gray"><o:p></o:p></span></span></p>
<p class="MsoNormal"><span style="color:#A6A6A6;mso-fareast-language:NL"><o:p> </o:p></span></p>
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