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<p class="MsoNormal">Hi Roman,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The first thing I thought of is viral mini-genomes like the RNA in your MS2 paper.<o:p></o:p></p>
<p class="MsoNormal">I have seen viruses stripped off their capsids like that before – clustered RNA has high contrast, in particular at 200 kV or lower.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">You could run a gel and stain for nucleic acid, but I guess you already know more… ;-)<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best regards,<o:p></o:p></p>
<p class="MsoNormal"> Matthias<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> <b>On Behalf Of
</b>r.i.koning@lumc.nl<br>
<b>Sent:</b> Monday, October 10, 2022 9:46 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] unknown structures<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dear all,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I would like to have you expert opinion on what the structures in the attached image might be. The structures are quite dense in my opinion. I have my ideas but I am not at all sure. I hope any of you have seen similar structures.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best regards,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Roman <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">Dr. R.I.Koning<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:8.0pt;color:gray;mso-fareast-language:NL"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-GB" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">Leiden University Medical Center, Cell and Chemical Biology, Electron Microsc</span><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">opy</span><span style="font-size:8.0pt;color:gray;mso-fareast-language:NL"><o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">visiting address.</span></b><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">
Einthovenweg 20, Building 2, zone S1-P, room R-90-21<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">tel.</span></b><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">
(31) 71 526 9296 <b>fax.</b> (+31) 71 526 8270 <b>mail. </b></span><a href="mailto:r.i.koning@lumc.nl"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:blue;mso-fareast-language:NL">r.i.koning@lumc.nl</span></a><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:gray;mso-fareast-language:NL">
<b>web.</b> </span><a href="https://urldefense.com/v3/__http:/www.kosterlab.nl/__;!!Mih3wA!F4lNir_quXjQA0OCOnJTcn8SALwmpJlIYa7sS_mNNlUXvFr8spA-OUWkVBosGs3LAI6wkv09XnIncfYrM5h64LI$"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:blue;mso-fareast-language:NL">www.kosterlab.nl</span></a><span style="font-size:8.0pt;font-family:"Times New Roman",serif;color:gray;mso-fareast-language:NL"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="NL" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#A6A6A6;mso-fareast-language:NL"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="NL"><o:p> </o:p></span></p>
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