[3dem] tilted transfer functions?

[Sjors Scheres]

Hi David,

If you do your subtomogram averaging in relion-4, estimating these 
high-tilt CTFs over the entire micrographs is probably not that 
important, provided that you optimise defoci in a CTF refinement job 
after averaging. There, the entire tilt and sample geometry is taken 
into account.

HTH,

Sjors


On 28/02/2022 22:41, Morgan, David Gene wrote:
> Hi,
>
> What's the best way to evaluate the CTF of an image that has a 
> significant amount of tilt?
>
> -- 
>     politics is more difficult than physics.
>                                              A. Einstein
>
>             David Gene Morgan
>         Electron Microscopy Center
>              047E Simon Hall
>              IU Bloomington
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