[3dem] 3dem Digest, Vol 178, Issue 12
Guobin Hu
gb_hu at yahoo.com
Mon Jun 13 13:10:22 PDT 2022
Thanks Winnie! Will check it out.
Sent from my iPhone, please excuse typos
> On Jun 13, 2022, at 4:05 PM, Winnie Liang <winnie9388 at gmail.com> wrote:
>
> Neptune fluid flow systems sells manual plungers. If you are interested, please contact Trevor McQueen at neptuneffs at gmail.com.
>
> Best,
>
> Winnie
>
> Sent from my iPhone
>
>> On Jun 13, 2022, at 3:00 PM, 3dem-request at ncmir.ucsd.edu wrote:
>>
>> Send 3dem mailing list submissions to
>> 3dem at ncmir.ucsd.edu
>>
>> To subscribe or unsubscribe via the World Wide Web, visit
>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>> or, via email, send a message with subject or body 'help' to
>> 3dem-request at ncmir.ucsd.edu
>>
>> You can reach the person managing the list at
>> 3dem-owner at ncmir.ucsd.edu
>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of 3dem digest..."
>>
>>
>> Today's Topics:
>>
>> 1. Manual Cryo-plungers (Guobin Hu)
>> 2. Re: Manual Cryo-plungers (Sylvain Trepout)
>> 3. Re: Manual Cryo-plungers (Guobin Hu)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Sun, 12 Jun 2022 15:15:18 -0400
>> From: Guobin Hu <gb_hu at yahoo.com>
>> To: 3dem at ncmir.ucsd.edu
>> Subject: [3dem] Manual Cryo-plungers
>> Message-ID: <0E31F44D-5A58-426A-8DB0-AD56F5E99811 at yahoo.com>
>> Content-Type: text/plain; charset=us-ascii
>>
>> Dear Cryo-EM folks,
>>
>> We are looking for a manual Cryo-plunger. Any recommendation of commercial plungers would be appreciated.
>>
>> Thanks,
>> Guobin
>>
>> Sent from my iPhone, please excuse typos
>>
>>
>> ------------------------------
>>
>> Message: 2
>> Date: Mon, 13 Jun 2022 08:58:02 +1000
>> From: Sylvain Trepout <sylvain.trepout at monash.edu>
>> To: Guobin Hu <gb_hu at yahoo.com>
>> Cc: 3dem at ncmir.ucsd.edu
>> Subject: Re: [3dem] Manual Cryo-plungers
>> Message-ID:
>> <CAGOVMwEV7ziLP2jKt1FRUoSOZ=i_JGPPu36cyz8LMT4hUbe6Mg at mail.gmail.com>
>> Content-Type: text/plain; charset="utf-8"
>>
>> Dear Guobin,
>>
>> The Leica EM-CPC is a pretty good one.
>> It is a manual plunge-freezer but the liquid nitrogen refilling is
>> automatic (which allows you to focus more on the grid preparation).
>> Actually, I am surprised that the fully-automatic plungers do not have
>> this, even as an option, because this is so convenient.
>> You can also control the temperature quite accurately.
>> I used it for more than a decade and I was pretty happy with it.
>> I think this is a discontinued product, but you might be able to get one
>> second-hand since now everyone goes for fully-automated ones.
>>
>> Regards,
>> Sylvain
>>
>> PhD
>> Research Fellow - Microscopy Australia
>>
>> *Monash University*
>> Ramaciotti Centre for Cryo-EM
>> G92, 15 Innovation Walk, Clayton Campus
>> Victoria, 3800 Australia
>>
>> E: sylvain.trepout at monash.edu
>>
>>
>>>> On Mon, 13 Jun 2022 at 05:15, Guobin Hu <gb_hu at yahoo.com> wrote:
>>>
>>> Dear Cryo-EM folks,
>>>
>>> We are looking for a manual Cryo-plunger. Any recommendation of commercial
>>> plungers would be appreciated.
>>>
>>> Thanks,
>>> Guobin
>>>
>>> Sent from my iPhone, please excuse typos
>>> _______________________________________________
>>> 3dem mailing list
>>> 3dem at ncmir.ucsd.edu
>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>>>
>> -------------- next part --------------
>> An HTML attachment was scrubbed...
>> URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20220613/e6b2f656/attachment-0001.html>
>>
>> ------------------------------
>>
>> Message: 3
>> Date: Mon, 13 Jun 2022 10:10:16 -0400
>> From: Guobin Hu <gb_hu at yahoo.com>
>> To: Sylvain Trepout <sylvain.trepout at monash.edu>
>> Cc: 3dem at ncmir.ucsd.edu
>> Subject: Re: [3dem] Manual Cryo-plungers
>> Message-ID: <3C73D827-81E9-4ACF-AE02-D5F4869B55FA at yahoo.com>
>> Content-Type: text/plain; charset="utf-8"
>>
>> Dear Sylvain,
>>
>> Thanks a lot for your input! It is very useful. The only concern is it would be hard to find solutions for a discontinued product when issues arise. Hopefully there are more options in the community.
>>
>> Best,
>> Guobin
>>
>>
>>
>> Sent from my iPhone, please excuse typos
>>
>>>> On Jun 12, 2022, at 6:58 PM, Sylvain Trepout <sylvain.trepout at monash.edu> wrote:
>>>
>>> ?
>>> Dear Guobin,
>>>
>>> The Leica EM-CPC is a pretty good one.
>>> It is a manual plunge-freezer but the liquid nitrogen refilling is automatic (which allows you to focus more on the grid preparation).
>>> Actually, I am surprised that the fully-automatic plungers do not have this, even as an option, because this is so convenient.
>>> You can also control the temperature quite accurately.
>>> I used it for more than a decade and I was pretty happy with it.
>>> I think this is a discontinued product, but you might be able to get one second-hand since now everyone goes for fully-automated ones.
>>>
>>> Regards,
>>> Sylvain
>>> PhD
>>> Research Fellow - Microscopy Australia
>>> Monash University
>>> Ramaciotti Centre for Cryo-EM
>>> G92, 15 Innovation Walk, Clayton Campus
>>> Victoria, 3800 Australia
>>> E: sylvain.trepout at monash.edu
>>>
>>>
>>>>> On Mon, 13 Jun 2022 at 05:15, Guobin Hu <gb_hu at yahoo.com> wrote:
>>>> Dear Cryo-EM folks,
>>>>
>>>> We are looking for a manual Cryo-plunger. Any recommendation of commercial plungers would be appreciated.
>>>>
>>>> Thanks,
>>>> Guobin
>>>>
>>>> Sent from my iPhone, please excuse typos
>>>> _______________________________________________
>>>> 3dem mailing list
>>>> 3dem at ncmir.ucsd.edu
>>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>> -------------- next part --------------
>> An HTML attachment was scrubbed...
>> URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20220613/d8bede8f/attachment-0001.html>
>>
>> ------------------------------
>>
>> Subject: Digest Footer
>>
>> _______________________________________________
>> 3dem mailing list
>> 3dem at ncmir.ucsd.edu
>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>>
>>
>> ------------------------------
>>
>> End of 3dem Digest, Vol 178, Issue 12
>> *************************************
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
More information about the 3dem
mailing list