[3dem] 3dem Digest, Vol 178, Issue 12

[Winnie Liang]

Neptune fluid flow systems sells manual plungers.  If you are interested, please contact Trevor McQueen at neptuneffs at gmail.com.  

Best,

Winnie 

Sent from my iPhone

> On Jun 13, 2022, at 3:00 PM, 3dem-request at ncmir.ucsd.edu wrote:
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> Today's Topics:
> 
>   1. Manual Cryo-plungers (Guobin Hu)
>   2. Re: Manual Cryo-plungers (Sylvain Trepout)
>   3. Re: Manual Cryo-plungers (Guobin Hu)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Sun, 12 Jun 2022 15:15:18 -0400
> From: Guobin Hu <gb_hu at yahoo.com>
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] Manual Cryo-plungers
> Message-ID: <0E31F44D-5A58-426A-8DB0-AD56F5E99811 at yahoo.com>
> Content-Type: text/plain; charset=us-ascii
> 
> Dear Cryo-EM  folks, 
> 
> We are looking for a manual Cryo-plunger. Any recommendation of commercial plungers would be appreciated. 
> 
> Thanks,
> Guobin
> 
> Sent from my iPhone, please excuse typos
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Mon, 13 Jun 2022 08:58:02 +1000
> From: Sylvain Trepout <sylvain.trepout at monash.edu>
> To: Guobin Hu <gb_hu at yahoo.com>
> Cc: 3dem at ncmir.ucsd.edu
> Subject: Re: [3dem] Manual Cryo-plungers
> Message-ID:
>    <CAGOVMwEV7ziLP2jKt1FRUoSOZ=i_JGPPu36cyz8LMT4hUbe6Mg at mail.gmail.com>
> Content-Type: text/plain; charset="utf-8"
> 
> Dear Guobin,
> 
> The Leica EM-CPC is a pretty good one.
> It is a manual plunge-freezer but the liquid nitrogen refilling is
> automatic (which allows you to focus more on the grid preparation).
> Actually, I am surprised that the fully-automatic plungers do not have
> this, even as an option, because this is so convenient.
> You can also control the temperature quite accurately.
> I used it for more than a decade and I was pretty happy with it.
> I think this is a discontinued product, but you might be able to get one
> second-hand since now everyone goes for fully-automated ones.
> 
> Regards,
> Sylvain
> 
> PhD
> Research Fellow - Microscopy Australia
> 
> *Monash University*
> Ramaciotti Centre for Cryo-EM
> G92, 15 Innovation Walk, Clayton Campus
> Victoria, 3800 Australia
> 
> E: sylvain.trepout at monash.edu
> 
> 
>> On Mon, 13 Jun 2022 at 05:15, Guobin Hu <gb_hu at yahoo.com> wrote:
>> 
>> Dear Cryo-EM  folks,
>> 
>> We are looking for a manual Cryo-plunger. Any recommendation of commercial
>> plungers would be appreciated.
>> 
>> Thanks,
>> Guobin
>> 
>> Sent from my iPhone, please excuse typos
>> _______________________________________________
>> 3dem mailing list
>> 3dem at ncmir.ucsd.edu
>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>> 
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> ------------------------------
> 
> Message: 3
> Date: Mon, 13 Jun 2022 10:10:16 -0400
> From: Guobin Hu <gb_hu at yahoo.com>
> To: Sylvain Trepout <sylvain.trepout at monash.edu>
> Cc: 3dem at ncmir.ucsd.edu
> Subject: Re: [3dem] Manual Cryo-plungers
> Message-ID: <3C73D827-81E9-4ACF-AE02-D5F4869B55FA at yahoo.com>
> Content-Type: text/plain; charset="utf-8"
> 
> Dear Sylvain,
> 
> Thanks a lot for your input! It is very useful. The only concern is it would be hard to find solutions for a discontinued product when issues arise. Hopefully there are more options in the community. 
> 
> Best,
> Guobin
> 
> 
> 
> Sent from my iPhone, please excuse typos
> 
>> On Jun 12, 2022, at 6:58 PM, Sylvain Trepout <sylvain.trepout at monash.edu> wrote:
>> 
>> ?
>> Dear Guobin,
>> 
>> The Leica EM-CPC is a pretty good one.
>> It is a manual plunge-freezer but the liquid nitrogen refilling is automatic (which allows you to focus more on the grid preparation).
>> Actually, I am surprised that the fully-automatic plungers do not have this, even as an option, because this is so convenient.
>> You can also control the temperature quite accurately.
>> I used it for more than a decade and I was pretty happy with it.
>> I think this is a discontinued product, but you might be able to get one second-hand since now everyone goes for fully-automated ones.
>> 
>> Regards,
>> Sylvain
>> PhD
>> Research Fellow - Microscopy Australia
>> Monash University
>> Ramaciotti Centre for Cryo-EM
>> G92, 15 Innovation Walk, Clayton Campus
>> Victoria, 3800 Australia
>> E: sylvain.trepout at monash.edu
>> 
>> 
>>>> On Mon, 13 Jun 2022 at 05:15, Guobin Hu <gb_hu at yahoo.com> wrote:
>>> Dear Cryo-EM  folks, 
>>> 
>>> We are looking for a manual Cryo-plunger. Any recommendation of commercial plungers would be appreciated. 
>>> 
>>> Thanks,
>>> Guobin
>>> 
>>> Sent from my iPhone, please excuse typos
>>> _______________________________________________
>>> 3dem mailing list
>>> 3dem at ncmir.ucsd.edu
>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
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> End of 3dem Digest, Vol 178, Issue 12
> *************************************