[3dem] Manual Cryo-plungers

[Guobin Hu]

Dear Sylvain,

Thanks a lot for your input! It is very useful. The only concern is it would be hard to find solutions for a discontinued product when issues arise. Hopefully there are more options in the community. 

Best,
Guobin



Sent from my iPhone, please excuse typos

> On Jun 12, 2022, at 6:58 PM, Sylvain Trepout <sylvain.trepout at monash.edu> wrote:
> 
> 
> Dear Guobin,
> 
> The Leica EM-CPC is a pretty good one.
> It is a manual plunge-freezer but the liquid nitrogen refilling is automatic (which allows you to focus more on the grid preparation).
> Actually, I am surprised that the fully-automatic plungers do not have this, even as an option, because this is so convenient.
> You can also control the temperature quite accurately.
> I used it for more than a decade and I was pretty happy with it.
> I think this is a discontinued product, but you might be able to get one second-hand since now everyone goes for fully-automated ones.
> 
> Regards,
> Sylvain
> PhD
> Research Fellow - Microscopy Australia
> Monash University
> Ramaciotti Centre for Cryo-EM
> G92, 15 Innovation Walk, Clayton Campus
> Victoria, 3800 Australia
> E: sylvain.trepout at monash.edu
> 
> 
>> On Mon, 13 Jun 2022 at 05:15, Guobin Hu <gb_hu at yahoo.com> wrote:
>> Dear Cryo-EM  folks, 
>> 
>> We are looking for a manual Cryo-plunger. Any recommendation of commercial plungers would be appreciated. 
>> 
>> Thanks,
>> Guobin
>> 
>> Sent from my iPhone, please excuse typos
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