[3dem] low-humidity workstation (glovebox)? Any experience?

[Lars-Anders Carlson]

Hi Tobias,
You might want to look at the work of your Max Planck colleagues in 
Dortmund (Sebastian Tacke et al in the Raunser group). See Fig 2 in this 
paper which is also on biorxiv:

https://urldefense.com/v3/__https://doi.org/10.1016/j.jsb.2021.107743__;!!Mih3wA!AM-SwrN3jrAIRdM8UT6LECX02tHnlsA5HIuG6Vz98KcrdUFfAEXmRCUM-1Nc6Tld5VmAPbvEJF09tas-oABdIwzslJ1hKQ$ 

Cheers,
Lars



On 2022-07-28 15:05, Tobias Furstenhaupt wrote:
> Dear colleagues,
> 
> we have a dedicated cryo prep room that has a controlled (low) humidty. We will use it to clip autogrids and the room contains also our Cryo-LM. But we want to reduce potential contamination on grids during preparation even more and thought of an (ownbuilt) glovebox (something like in the attached image). An enclosure is one part - but flooding it with 'dry' gas is another question.
> Does anybody of you have something installed and what is your experience? We want to keep it as simple (and cheap) as possible? Is it worth the effort? What gas you use to flood it? Do you try to dry it somehow?
>   
> thanks alot in advance
> Tobias
> 
> 
> -----------------------------------
> Tobias Fürstenhaupt, PhD
> head of Electron Microscopy
> Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
> Pfotenhauerstrasse 108
> 01307 Dresden, Germany
> 
> mail: furstenh at mpi-cbg.de
> phone: (+49) (0)351 210-2690
> cell: (+49) (0)176 / 44498706 (NEW!)
> 
> 
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem

-- 
Lars-Anders Carlson
Assistant Professor
Dept of Medical Biochemistry and Biophysics
Wallenberg Centre for Molecular Medicine
Molecular Infection Medicine Sweden
Umeå University
901 87 Umeå, Sweden
https://urldefense.com/v3/__http://www.carlsonlab.se/__;!!Mih3wA!AM-SwrN3jrAIRdM8UT6LECX02tHnlsA5HIuG6Vz98KcrdUFfAEXmRCUM-1Nc6Tld5VmAPbvEJF09tas-oABdIwx1dteg7A$