[3dem] low-humidity workstation (glovebox)? Any experience?

[Ludtke, Steven J.]

What sort of contamination are you hoping to avoid, and for what processes?  Most people plasma clean or glow discharge their grids prior to use, which will pretty effectively remove most non-ice "contamination". If you mean ice contamination, clearly that isn't something that will occur until after vitrification, so are you talking about moving the clipping process completely into a glove box? Are you doing some other sort of specimen preparation?

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Steven Ludtke, Ph.D. <sludtke at bcm.edu<mailto:sludtke at bcm.edu>>                      Baylor College of Medicine
Charles C. Bell Jr., Professor of Structural Biology        Dept. of Biochemistry
Deputy Director, Advanced Technical Cores                   and Molecular Biology
Academic Director, CryoEM Core
Co-Director CIBR Center



On Jul 28, 2022, at 8:05 AM, Tobias Furstenhaupt <furstenh at mpi-cbg.de<mailto:furstenh at mpi-cbg.de>> wrote:

***CAUTION:***  This email is not from a BCM Source.  Only click links or open attachments you know are safe.

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Dear colleagues,

we have a dedicated cryo prep room that has a controlled (low) humidty. We will use it to clip autogrids and the room contains also our Cryo-LM. But we want to reduce potential contamination on grids during preparation even more and thought of an (ownbuilt) glovebox (something like in the attached image). An enclosure is one part - but flooding it with 'dry' gas is another question.
Does anybody of you have something installed and what is your experience? We want to keep it as simple (and cheap) as possible? Is it worth the effort? What gas you use to flood it? Do you try to dry it somehow?

thanks alot in advance
Tobias


-----------------------------------
Tobias Fürstenhaupt, PhD
head of Electron Microscopy
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstrasse 108
01307 Dresden, Germany

mail: furstenh at mpi-cbg.de<mailto:furstenh at mpi-cbg.de>
phone: (+49) (0)351 210-2690
cell: (+49) (0)176 / 44498706 (NEW!)
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