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What sort of contamination are you hoping to avoid, and for what processes? Most people plasma clean or glow discharge their grids prior to use, which will pretty effectively remove most non-ice "contamination". If you mean ice contamination, clearly that
isn't something that will occur until after vitrification, so are you talking about moving the clipping process completely into a glove box? Are you doing some other sort of specimen preparation?
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<div style="margin: 0px; font-stretch: normal; line-height: normal;" class=""><span style="font-variant-ligatures: no-common-ligatures;" class="">--------------------------------------------------------------------------------------</span></div>
<div style="margin: 0px; font-stretch: normal; line-height: normal;" class=""><span style="font-variant-ligatures: no-common-ligatures;" class="">Steven Ludtke, Ph.D. <<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a>> Baylor
College of Medicine</span></div>
<div style="margin: 0px; font-stretch: normal; line-height: normal;" class=""><span style="font-variant-ligatures: no-common-ligatures;" class="">Charles C. Bell Jr., Professor of Structural Biology </span>Dept. of Biochemistry </div>
<div style="margin: 0px; font-stretch: normal; line-height: normal;" class="">Deputy Director, Advanced Technical Cores and Molecular Biology</div>
<div style="margin: 0px; font-stretch: normal; line-height: normal;" class=""><span style="font-variant-ligatures: no-common-ligatures;" class="">Academic Director, CryoEM Core</span></div>
<div style="margin: 0px; font-stretch: normal; line-height: normal;" class=""><span style="font-variant-ligatures: no-common-ligatures;" class="">Co-Director CIBR Center</span></div>
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<div class="">On Jul 28, 2022, at 8:05 AM, Tobias Furstenhaupt <<a href="mailto:furstenh@mpi-cbg.de" class="">furstenh@mpi-cbg.de</a>> wrote:</div>
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<div class="">***CAUTION:*** This email is not from a BCM Source. Only click links or open attachments you know are safe.<br class="">
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Dear colleagues,<br class="">
<br class="">
we have a dedicated cryo prep room that has a controlled (low) humidty. We will use it to clip autogrids and the room contains also our Cryo-LM. But we want to reduce potential contamination on grids during preparation even more and thought of an (ownbuilt)
glovebox (something like in the attached image). An enclosure is one part - but flooding it with 'dry' gas is another question.
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Does anybody of you have something installed and what is your experience? We want to keep it as simple (and cheap) as possible? Is it worth the effort? What gas you use to flood it? Do you try to dry it somehow?<br class="">
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thanks alot in advance<br class="">
Tobias <br class="">
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----------------------------------- <br class="">
Tobias Fürstenhaupt, PhD <br class="">
head of Electron Microscopy <br class="">
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) <br class="">
Pfotenhauerstrasse 108 <br class="">
01307 Dresden, Germany <br class="">
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mail: <a href="mailto:furstenh@mpi-cbg.de" class="">furstenh@mpi-cbg.de</a> <br class="">
phone: (+49) (0)351 210-2690 <br class="">
cell: (+49) (0)176 / 44498706 (NEW!)<br class="">
<span id="cid:7697C819-14A4-476B-B317-49FF4AF1E99E"><glovebox.jpg></span>_______________________________________________<br class="">
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