[3dem] Cryo-EM scientist / research engineer permanent position open at the CEA/IBS in Grenoble

[schoehn]

Dear Colleagues,

There is a permanent position open at the CEA Grenoble for a cryo-EM expert.

Description of the offer

To support the integrated structural biology programs carried out at 
DBSCI#, IBS (Structural Biology Institute) operates a number of 
state-of-the-art platforms that are accessible via the local PSB 
("Partnership for Structural Biology"; https://urldefense.proofpoint.com/v2/url?u=https-3A__www.psb-2Dgrenoble.eu_&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=EXHAmzc3IAA9fS4PXbBCrxI0e3-Lv9hnzQenZ0gyvZw&e= ), 
national (FRISBI) and European (Instruct-ERIC) programs.

In particular, the IBS operates an electron microscopy platform equipped 
with three electron microscopes: T12 for negative staining and cellular 
electron microscopy at room temperature; F20 for negative staining, 
cryo-EM, cellular electron microscopy at room temperature and in cryo 
mode, electron micro-diffraction; Glacios equipped with 2 direct 
electron detectors: K2 summit and Falcon 2. The latter instrument allows 
extensive screening of grids prior to the collection of high-resolution 
data both for the analysis of isolated particles and for cryo-electron 
tomography on isolated particles or cellular sections. The platform is 
also equipped with all the auxiliary instruments necessary for the 
preparation of grids in negative staining and cryo-EM, and also has 
access to an EMBL-IBS computing cluster for image analysis (660 CPUs; 40 
GPUs). In addition to operating its own electron microscopy platform, 
the IBS is also involved in the operation of the Krios CM01 electron 
microscopy platform at ESRF. The installation of a second 
state-of-the-art 300 kV microscope at ESRF (CM02) is expected to be 
completed in 2022 or beginning of 2023. CM02 will be operated by IBS 
staff and will provide access to a state-of-the-art instrument for 
national and international users (ESRF/ FRISBI/Instruct-ERIC).

The CEA is recruiting a scientist / reserach engineer on a permanent 
basis to support the cryo-electron microscopy projects within the DBSCI, 
the candidate must be a recognized expert in cryo-electron microscopy.

The position will be based at IBS, but the candidate will be expected to 
interact with the LPCV and IBS groups and to work on the CM02 platform 
at ESRF providing user support. The position is attached to the IBS MEM 
group, which operates the IBS electron microscopy platform 
(https://urldefense.proofpoint.com/v2/url?u=https-3A__www.ibs.fr_research_research-2Dgroups_methods-2Dand-2Delectron-2Dmicroscopy-2Dgroup_&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=uAx9WNj4LNRGvWATE3EJYUiL93Dxa1yry2zZar3ptdI&e= ) 
and will be in charge of the CM02 platform. The successful candidate 
will work under the responsibility of the Head of the Methods and 
Electron Microscopy Group.

The main activities of the successful candidate will be the following:

To be part of a scientific committee in charge of selecting DBSCI 
projects to be supported in cryo-electron microscopy (isolated particles 
and tomography).

Support and conduct the selected cryo-EM "single particle" projects, in 
close collaboration with the DBSCI research groups, from cryo-EM data 
collection to image analysis, 3D reconstructions and model building.

Support the selected cryo-electron tomography projects within the DBSCI.

Implementing the latest developments in image analysis to make the most 
of EM images, including the description of heterogeneous conformational 
ensembles.

Work closely with DBSCI staff involved in the projects and contribute to 
their training in cryo-electron microscopy data collection and analysis.

Participate in the operation of CM02 at ESRF (20% of time).

To develop his/her own research project in the framework of the research 
themes in progress at IBS or LPCV (30%).

The candidate must hold a PhD in structural biology, preferably in the 
field of electron microscopy, and have at least three years of 
postdoctoral experience in cryo-electron microscopy, validated by 
publications.

The position requires excellent skills in project management, protocol 
writing and scientific writing support (papers and grant applications). 
In addition, the candidate must have pedagogical skills to provide 
training to users.

We are looking for an enthusiastic candidate with excellent team spirit 
and good communication skills to support and lead collaborative projects.

In addition to proven expertise in all aspects of protein cryo-electron 
microscopy (grid preparation, data collection, image analysis, software 
expertise in Relion, Cryosparc, Cryolo, Linux environment), knowledge in 
protein biochemistry and/or in the development of sophisticated image 
analysis protocols to isolate rare conformations in silico would be a plus.

In line with the CEA's commitment to the integration of people with 
disabilities, this application is open to all.

For all enquiries about the position and application procedures, please 
contact the head of the MEM group: Guy Schoehn guy.schoehn at ibs.fr

  Sincerely yours


Guy Schoehn


# The IBS is a joint research unit (UMR) of the CEA, the CNRS and the 
University of Grenoble Alpes. Within the Grenoble Interdisciplinary 
Research Institute (IRIG), IBS forms with the LPCV, the Department of 
Integrated Structural and Cellular Biology (DBSCI). The IBS is located 
on the European Photon and Neutron (EPN) campus, with its international 
partners ESRF, EMBL and ILL. The IBS ( about 300 people) is using 
integrated structural biology approaches to focus on three main research 
programs (https://urldefense.proofpoint.com/v2/url?u=https-3A__www.ibs.fr_about-2Dus_science-2Dat-2Da-2Dglance_&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=8GQiY0K-4_1e8kHKCiU0wp5iM_H336m2OsK-xAyCO3k&e= ). The LPCV is 
located on the CEA campus in Grenoble. The LPCV (110 people) focuses on 
the study of plant biology (https://urldefense.proofpoint.com/v2/url?u=https-3A__www.lpcv.fr_en&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=R97JQDjxbtfZ7bxH-9yVL7IwtKLNiApYQdvtrxeLAlA&e= ).
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