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<p>Dear Colleagues,</p>
<p>There is a permanent position open at the CEA Grenoble for a
cryo-EM expert.<br>
</p>
<p>
</p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">Description of the
offer</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">To support the
integrated structural biology
programs carried out at DBSCI#, IBS (Structural Biology
Institute) operates a number of state-of-the-art
platforms that are accessible via the local PSB ("Partnership
for
Structural Biology"; <a class="moz-txt-link-freetext" href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.psb-2Dgrenoble.eu_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=EXHAmzc3IAA9fS4PXbBCrxI0e3-Lv9hnzQenZ0gyvZw&e=">https://www.psb-grenoble.eu/</a>), national
(FRISBI) and
European (Instruct-ERIC) programs.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">In particular, the
IBS operates an electron
microscopy platform equipped with three electron microscopes:
T12 for negative
staining and cellular electron microscopy at room temperature;
F20 for negative
staining, cryo-EM, cellular electron microscopy at room
temperature and in cryo
mode, electron micro-diffraction; Glacios equipped with 2 direct
electron
detectors: K2 summit and Falcon 2. The latter instrument allows
extensive
screening of grids prior to the collection of high-resolution
data both for the
analysis of isolated particles and for cryo-electron tomography
on isolated particles
or cellular sections. The platform is also equipped with all the
auxiliary
instruments necessary for the preparation of grids in negative
staining and
cryo-EM, and also has access to an EMBL-IBS computing cluster
for image
analysis (660 CPUs; 40 GPUs). In addition to operating its own
electron
microscopy platform, the IBS is also involved in the operation
of the Krios
CM01 electron microscopy platform at ESRF. The installation of a
second
state-of-the-art 300 kV microscope at ESRF (CM02) is expected to
be completed
in 2022 or beginning of 2023. CM02 will be operated by IBS staff
and will
provide access to a state-of-the-art instrument for national and
international
users (ESRF/ FRISBI/Instruct-ERIC).</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">The CEA is
recruiting a scientist / reserach engineer on a
permanent basis to support the cryo-electron microscopy projects
within the
DBSCI, the candidate must be a recognized expert in
cryo-electron microscopy.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">The position will
be based at IBS, but the
candidate will be expected to interact with the LPCV and IBS
groups and to work
on the CM02 platform at ESRF providing user support. The
position is attached
to the IBS MEM group, which operates the IBS electron microscopy
platform
(<a class="moz-txt-link-freetext" href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.ibs.fr_research_research-2Dgroups_methods-2Dand-2Delectron-2Dmicroscopy-2Dgroup_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=uAx9WNj4LNRGvWATE3EJYUiL93Dxa1yry2zZar3ptdI&e=">https://www.ibs.fr/research/research-groups/methods-and-electron-microscopy-group/</a>)
and will be in charge of the CM02 platform. The successful
candidate will work
under the responsibility of the Head of the Methods and Electron
Microscopy
Group.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">The main activities
of the successful candidate will be
the following: <br>
</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes"> </span>To be
part of a scientific committee in charge of selecting DBSCI
projects to be
supported in cryo-electron microscopy (isolated particles and
tomography).</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes">
</span>Support and conduct the selected cryo-EM "single
particle"
projects, in close collaboration with the DBSCI research groups,
from cryo-EM
data collection to image analysis, 3D reconstructions and model
building.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes">
</span>Support the selected cryo-electron tomography projects
within the DBSCI. </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes">
</span>Implementing the latest developments in image analysis to
make the most
of EM images, including the description of heterogeneous
conformational
ensembles.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes"> </span>Work
closely with DBSCI staff involved in the projects and contribute
to their
training in cryo-electron microscopy data collection and
analysis.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes">
</span>Participate in the operation of CM02 at ESRF (20% of
time).</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes"> </span>To
develop his/her own research project in the framework of the
research themes in
progress at IBS or LPCV (30%).</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><span
style="mso-spacerun:yes"> </span></span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">The candidate must
hold a PhD in structural
biology, preferably in the field of electron microscopy, and
have at least
three years of postdoctoral experience in cryo-electron
microscopy, validated
by publications.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">The position
requires excellent skills in
project management, protocol writing and scientific writing
support (papers and
grant applications). In addition, the candidate must have
pedagogical skills to
provide training to users.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">We are looking for
an enthusiastic candidate
with excellent team spirit and good communication skills to
support and lead
collaborative projects.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">In addition to
proven expertise in all aspects
of protein cryo-electron microscopy (grid preparation, data
collection, image
analysis, software expertise in Relion, Cryosparc, Cryolo, Linux
environment),
knowledge in protein biochemistry and/or in the development of
sophisticated
image analysis protocols to isolate rare conformations in silico
would be a
plus. </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">In line with the
CEA's commitment to the
integration of people with disabilities, this application is
open to all.</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">For all enquiries
about the position and
application procedures, please contact the head of the MEM
group: Guy Schoehn <a href="mailto:guy.schoehn@ibs.fr"
class="moz-txt-link-freetext">guy.schoehn@ibs.fr</a></span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> </span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"> Sincerely yours</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><br>
</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US">Guy Schoehn</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"><br>
</span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="mso-ansi-language:EN-US" lang="EN-US"># The IBS is a
joint research unit (UMR) of the
CEA, the CNRS and the University of Grenoble Alpes. Within the
Grenoble
Interdisciplinary Research Institute (IRIG), IBS forms with the
LPCV, the
Department of Integrated Structural and Cellular Biology
(DBSCI). The IBS is
located on the European Photon and Neutron (EPN) campus, with
its international
partners ESRF, EMBL and ILL. The IBS ( about 300 people) is
using integrated
structural biology approaches to focus on three main research
programs
(<a class="moz-txt-link-freetext" href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.ibs.fr_about-2Dus_science-2Dat-2Da-2Dglance_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=8GQiY0K-4_1e8kHKCiU0wp5iM_H336m2OsK-xAyCO3k&e=">https://www.ibs.fr/about-us/science-at-a-glance/</a>). The LPCV is
located on the
CEA campus in Grenoble. The LPCV (110 people) focuses on the
study of plant
biology (<a class="moz-txt-link-freetext" href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.lpcv.fr_en&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=X4JXSqf_ddnq4Ia2GibusJWGdqGT4qUwA1-S1JXmeAOM9J9wztMe74SIX_BW4PIT&s=R97JQDjxbtfZ7bxH-9yVL7IwtKLNiApYQdvtrxeLAlA&e=">https://www.lpcv.fr/en</a>). </span></p>
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