[3dem] 3d coordinates in relion 4
Pablo Conesa
pconesa at cnb.csic.es
Wed Feb 23 02:43:38 PST 2022
Hi, in the relion4 tutorial for tomagraphy a coordinates star file is
provided starting like this:
# version 30001 by xpytools
data_particles
loop_
_rlnTomoName #1
_rlnTomoParticleId #2
_rlnTomoManifoldIndex #3
_rlnCoordinateX #4
_rlnCoordinateY #5
_rlnCoordinateZ #6
_rlnOriginXAngst #7
_rlnOriginYAngst #8
_rlnOriginZAngst #9
_rlnAngleRot #10
_rlnAngleTilt #11
_rlnAnglePsi #12
_rlnClassNumber #13
_rlnRandomSubset #14
TS_01 1 1 644 1164 72 -0.000 -0.000 -0.000
-8.72 167.29 87.57 -1 2
TS_01 2 1 624 1112 64 -0.000 -0.000 -0.000
-130.65 175.35 84.29 -1 1
TS_01 3 1 676 1120 68 -0.000 -0.000 -0.000
-117.88 170.53 95.89 -1 2
TS_01 4 1 696 1168 84 -0.000 -0.000 -0.000
53.64 158.62 86.53 -1 1
TS_01 5 1 592 1156 72 -0.000 -0.000 -0.000
-78.74 169.66 78.39 -1 2
...
...
Could anyone let me know:
1.- _rlnCoordinateX y and Z are in pixels?
2.- are the coordinates picked on a binning 1 image set (1.35A/pixel)
3.- are relion coordinates referred to a "BOTTOM_LEFT" origin
4.- What was the thickness of the tomograms where the picking happened?
Thanks in advance,
--
Pablo Conesa - *Madrid Scipion <https://urldefense.proofpoint.com/v2/url?u=http-3A__scipion.i2pc.es&d=DwIDaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=mFXNrid_T1zGgJeYYid_PWwgqMEx_nRfYpn6B1j9QvKrVwpZMqVyDMevUCmS5Kkh&s=VPrtbQGHSiiGEYeAvUQ16vLOmkQQG0UBAW_bVvSSIKA&e= > team*
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