[3dem] Apoferritin for benchmarking

[Adam Schröfel]

Hello 3DEMers,
I am wondering if there is any reliable way to prepare apoferritin
particles for microscope benchmarking and acquisition tuning.
I've got a A3660 Apoferritin from the equine spleen from Sigma in 50%
glycerol which is probably the most common source. Is there any protocol on
how to process the protein? What buffer type I should use and how to get
rid of glycerol - dialysis, gel filtration? What is the best concentration
to use, the grid type, etc.? I read some protocols and papers, but the
processing relatively differs one from another or the protocol is not
perfectly clear to me.
Thanks for any suggestions!
Best regards

Adam

Adam Schröfel

Department of Cell Biology | Biocev

Charles University

Prumyslova 595, 252 50 Vestec

Prague West, Czech Republic

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