[3dem] Imaging hexagonal ice crystals (aka "contamination") deliberately

Jan Hübinger jan.huebinger at hotmail.de
Wed Sep 29 05:55:35 PDT 2021


Hi,

some years ago, we have been imaging ice crystals by cryoEM (no SPA) in aqueous media containing different cryoprotective additives after plunge freezing and subsequent defined warming steps.
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.sciencedirect.com_science_article_pii_S0006349515010000-3Fvia-253Dihub&d=DwIFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=fAzVnNGJDWbKgarjeGEb5dGdPIpChcGjogbOpPUHsFk&s=etFK446Ey_ehOhYslRxg7U0lndxJXLfIhkMpzHZhtHk&e= 
Maybe this helps as a starting point.

Best regards,
Jan

----
Dr. Jan Huebinger
Max-Planck-Institute of Molecular Physiology, Dortmund
jan.huebinger at mpi-dortmund.mpg.de
Phone: +49 231-133 2203


________________________________
Von: 3dem <3dem-bounces at ncmir.ucsd.edu> im Auftrag von t.sharp at lumc.nl <t.sharp at lumc.nl>
Gesendet: Dienstag, 28. September 2021 16:32
An: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Betreff: [3dem] Imaging hexagonal ice crystals (aka "contamination") deliberately


Hi all,


I'd like to image hexagonal ice crystals. I see them occasionally as contamination, but not reliably. Now I'd like to vitrify small crystals of hexagonal water ice within an amorphous medium and image them using cryoEM in an analogous way to how we do SPA of proteins. Has anyone done something similar before? My initial ideas were to use a different medium (maybe with high salt/glycerol) and then vitrify in a poor cryogen (e.g., liquid nitrogen), but I've not found any literature yet. Any and all tips welcome!


Thanks in advance for your suggestions,


Thom


Dr. Thomas H. Sharp

Assistant Professor

PI: Bio-Nanopatterning

* 0031-(0)-7152-69499

* t.sharp at lumc.nl<mailto:t.sharp at lumc.nl>

Website: https://urldefense.proofpoint.com/v2/url?u=https-3A__ccb.lumc.nl_about-2Dthe-2Dsharp-2Dlab-2D90&d=DwIFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=fAzVnNGJDWbKgarjeGEb5dGdPIpChcGjogbOpPUHsFk&s=pEpHJUOfxknSEkrjwjs1akE6uj3kxeF5zLLFijmfTNY&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ccb.lumc.nl_about-2Dthe-2Dsharp-2Dlab-2D90&d=DwMFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=-fx6OPS6KXgM3nes4KiZ13FVKhQYEdMmiQedVib53A8&s=88G21hW0ngyUZqBXMsGPsp8GVqRLkOnr0ovncR2Eok8&e=>

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