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Hi,</div>
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some years ago, we have been imaging ice crystals by cryoEM (no SPA) in aqueous media containing different cryoprotective additives after plunge freezing and subsequent defined warming steps. </div>
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<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.sciencedirect.com_science_article_pii_S0006349515010000-3Fvia-253Dihub&d=DwMFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=fAzVnNGJDWbKgarjeGEb5dGdPIpChcGjogbOpPUHsFk&s=etFK446Ey_ehOhYslRxg7U0lndxJXLfIhkMpzHZhtHk&e=" id="LPlnk">https://www.sciencedirect.com/science/article/pii/S0006349515010000?via%3Dihub</a></div>
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Maybe this helps as a starting point.</div>
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Best regards,</div>
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Jan</div>
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----</div>
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Dr. Jan Huebinger</div>
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Max-Planck-Institute of Molecular Physiology, Dortmund <br>
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jan.huebinger@mpi-dortmund.mpg.de</div>
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Phone: +49 231-133 2203<br>
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<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" face="Calibri, sans-serif" color="#000000"><b>Von:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> im Auftrag von t.sharp@lumc.nl <t.sharp@lumc.nl><br>
<b>Gesendet:</b> Dienstag, 28. September 2021 16:32<br>
<b>An:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Betreff:</b> [3dem] Imaging hexagonal ice crystals (aka "contamination") deliberately</font>
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<p style="margin-top: 0px; margin-bottom: 0px;">Hi all, </p>
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<p style="margin-top: 0px; margin-bottom: 0px;">I'd like to image hexagonal ice crystals. I see them occasionally as contamination, but not reliably. Now I'd like to vitrify small crystals of hexagonal water ice within an amorphous medium and image them using
cryoEM in an analogous way to how we do SPA of proteins. Has anyone done something similar before? My initial ideas were to use a different medium (maybe with high salt/glycerol) and then vitrify in a poor cryogen (e.g., liquid nitrogen), but I've not found
any literature yet. Any and all tips welcome!</p>
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<p style="margin-top: 0px; margin-bottom: 0px;">Thanks in advance for your suggestions,</p>
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<span style="color:rgb(54,95,145); font-size:10pt; font-family:Arial,Helvetica,sans-serif">Thom</span></p>
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