[3dem] Imaging hexagonal ice crystals (aka "contamination") deliberately

[Jacopo Marino]

Hi - that might not be straightforward because glycerol and liquid nitrogen can be used for obtaining vitreous ice:
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.biorxiv.org_content_10.1101_2021.09.10.459874v1&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=pkVWEdJVQvbN2lVtr-vpkY0E_vFhLN0AHkdObS3jOz4&s=69esJ6N23UCVmOcgtVqnFj7ge8xK-lkW8CF4wfVKopw&e= 
Cheers 
Jacopo 

Dr. Jacopo Marino Laboratory of Biomolecular Research Paul Scherrer Institute OFLB/005 5232 
Villigen PSI, Switzerland
tel: +41 56 310 5777
e-mail: jacopo.marino at psi.ch

> On 28 Sep 2021, at 16:32, <t.sharp at lumc.nl> <t.sharp at lumc.nl> wrote:
> 
> Hi all, 
> 
> 
> 
> I'd like to image hexagonal ice crystals. I see them occasionally as contamination, but not reliably. Now I'd like to vitrify small crystals of hexagonal water ice within an amorphous medium and image them using cryoEM in an analogous way to how we do SPA of proteins. Has anyone done something similar before? My initial ideas were to use a different medium (maybe with high salt/glycerol) and then vitrify in a poor cryogen (e.g., liquid nitrogen), but I've not found any literature yet. Any and all tips welcome!
> 
> 
> 
> Thanks in advance for your suggestions,
> 
> 
> 
> Thom
> 
> Dr. Thomas H. Sharp
> Assistant Professor
> PI: Bio-Nanopatterning
> ( 0031-(0)-7152-69499
> * t.sharp at lumc.nl
> Website: https://urldefense.proofpoint.com/v2/url?u=https-3A__ccb.lumc.nl_about-2Dthe-2Dsharp-2Dlab-2D90&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=pkVWEdJVQvbN2lVtr-vpkY0E_vFhLN0AHkdObS3jOz4&s=xDR6gZkUCIMXqf-qs58nC453_Lju1jGtpTyuOmU7X8k&e= 
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