[3dem] Imaging hexagonal ice crystals (aka "contamination") deliberately

[t.sharp at lumc.nl]

Hi all,


I'd like to image hexagonal ice crystals. I see them occasionally as contamination, but not reliably. Now I'd like to vitrify small crystals of hexagonal water ice within an amorphous medium and image them using cryoEM in an analogous way to how we do SPA of proteins. Has anyone done something similar before? My initial ideas were to use a different medium (maybe with high salt/glycerol) and then vitrify in a poor cryogen (e.g., liquid nitrogen), but I've not found any literature yet. Any and all tips welcome!


Thanks in advance for your suggestions,

Thom

Dr. Thomas H. Sharp
Assistant Professor
PI: Bio-Nanopatterning
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