[3dem] Postdoc position in cryoEM of GPCRs in France

[Anaïs Menny]

Hello everyone,

We are looking for an early career postdoctoral researcher to join our team
‘Neuroreceptors, dynamics and function
<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.igf.cnrs.fr_index.php_en_h-2Dteams-2Den_ht-2Drondard-2Den-23research-2Dsubject&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TBAC-IrVW6o6oVWyU6fc2pR2u756MqEOrGtVi9PSGBvTPn10Ukqij3sTRvDqjrh4&s=BAhSjO2onlQ926yNZI6i8npNCoiXZx18GOx_reSqvLY&e= >’
at the Institute of Functional Genomics in Montpellier, France. The *position
is for 30 months*, with an ideal start date in April 2022.


The recruited candidate will be working on *solving structures of GPCR
complexes using single particle cryoEM and investigating their interactions
with lipids.* You will have access to an EM facility housing a 200 KeV JEOL
with FEG, K3 camera and GIF, and a 120 keV JEOL for screenings. Your work
will also be supported by several high-end platforms covering GPCR
functional assays and light microscopy.



The ideal candidate should have:

-  A PhD, or about to be awarded, in biochemistry, biophysics or structural
biology with relevant associated publication(s).

- Experience with membrane proteins expression and purification is a must.
Specifically, experience in eukaryotic expression system (mammalian cells
and/or insect cells) will be greatly appreciated.

- Experience in cryoEM is desirable.

- Previous experience working with GPCRs will be appreciated but isn’t
essential.

- The ability to work collaboratively and be a team player is essential.
The candidate should also be able to work independently and efficiently.

- Good oral and written skills in English is also essential as all lab
business and meetings are conducted in English. French speaking is not
required.


Our team, led by Philippe Rondard/ Jean-Philippe Pin, is made up of 10+
researchers, postdocs, engineers and PhD students of different
nationalities forming a friendly and dynamic group. Our work mainly focuses
on the study of Class C GPCRs using innovative pharmacological tools such
as nanobodies and photoactivable ligands, as well as FRET to analyze GPCRs
function and conformations at the molecular and single molecule level
(collaboration with E Margeat, on campus).
The advertised position is funded by an ANR grant coordinated by Dr Anaïs
Menny, who will be your direct supervisor, dedicated to expanding our
research themes to single particle cryoEM and other biophysical approaches.
The scientific environment at the institute also provides ample interaction
possibilities and support from other teams studying GPCRs, ranging from
neuropharmacology to cryoEM and crystallography.


The city of Montpellier is particularly attractive with its very large
research community, dynamic cultural life, lots of bars and restaurants,
affordable housing and sun all year round. It is located 20 min from the
Mediterranean sea and is surrounded by vineyards and the hilly Cevennes.


*To apply, follow this link*: https://urldefense.proofpoint.com/v2/url?u=https-3A__bit.ly_3HiXA9q&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TBAC-IrVW6o6oVWyU6fc2pR2u756MqEOrGtVi9PSGBvTPn10Ukqij3sTRvDqjrh4&s=V__mDDbdRTCFPjbfrqunljZhcR5fyNalc74rnpq49PI&e= 


The candidate should provide a CV, a short cover letter describing their
scientific interests, and name and contact details of two references.


Please contact Anaïs Menny for any informal inquiries about the position:
anais.menny at igf.cnrs.fr



Relevant recent publications:

Allosteric modulators enhance agonist efficacy by increasing the residence
time of a GPCR in the active state. Cao AM*, Quast RB*, Fatemi F, Rondard
P, Pin JP, Margeat E. Nat Commun 2021. doi:10.1038/s41467-021-25620-5


Illuminating the allosteric modulation of the calcium-sensing receptor. Liu
H, Yi P, Zhao W, Wu Y,  Acher F, Pin JP, Liu J, Rondard P. PNAS September
1, 2020 117 (35) 21711-21722


Moreno Delgado D, Moller TC, Ster J, Giraldo J, Maurel D, Rovira X,
Scholler P, Zwier JM, Perroy J, Durroux T, Trinquet E, Prézeau L, Rondard P
and Pin J-P (2017) Pharmacological evidence for a metabotropic glutamate
receptor heterodimer in neuronal cells. Elife *6*: e25233.


Structural basis for how sMAC is packaged for clearance. Menny A*, Lukassen
MV*, Couves EC, Franc V, Heck AJR, Bubeck D. Nat Commun, 2021. doi:
10.1038/s41467-021-26366-w


CryoEM reveals how the complement membrane attack complex ruptures lipid
bilayers. Menny A, Serna M, Boyd CM, Gardner S, Joseph AP, Morgan BP, Topf
M, Brooks NJ, Bubeck D. Nat Commun, 2018. doi: 10.1038/s41467-018-07653-5

-- 
Anaïs Menny
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