<div dir="ltr"><div class="gmail_default" style="text-align:start;color:rgb(0,0,0)"><span style="text-align:justify"><font face="arial, helvetica, sans-serif">Hello everyone,<br></font></span><span style="font-family:Arial;text-align:justify"><br>We are looking for an early career postdoctoral researcher to join our team ‘</span><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.igf.cnrs.fr_index.php_en_h-2Dteams-2Den_ht-2Drondard-2Den-23research-2Dsubject&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TBAC-IrVW6o6oVWyU6fc2pR2u756MqEOrGtVi9PSGBvTPn10Ukqij3sTRvDqjrh4&s=BAhSjO2onlQ926yNZI6i8npNCoiXZx18GOx_reSqvLY&e=" style="font-family:Arial;text-align:justify"><span class="gmail-s2" style="font-kerning:none">Neuroreceptors, dynamics and function</span></a><span style="font-family:Arial;text-align:justify">’ at the Institute of Functional Genomics in Montpellier, France. The </span><b style="font-family:Arial;text-align:justify">position is for 30 months</b><span style="font-family:Arial;text-align:justify">, with an ideal start date in April 2022.</span></div><div class="gmail_default" style="font-family:arial,helvetica,sans-serif;color:#000000">
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none"><br>The recruited candidate will be working on <b>solving structures of GPCR complexes using single particle cryoEM and investigating their interactions with lipids.</b> You will have access to an EM facility housing a 200 KeV JEOL with FEG, K3 camera and GIF, and a 120 keV JEOL for screenings. Your work will also be supported by several high-end platforms covering GPCR functional assays and light microscopy.</span></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s2" style="text-decoration-line:underline;font-kerning:none">The ideal candidate should have:</span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">-<span class="gmail-Apple-converted-space">  </span>A PhD, or about to be awarded, in biochemistry, biophysics or structural biology with relevant associated publication(s).<span class="gmail-Apple-converted-space"> </span></span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">- Experience with membrane proteins expression and purification is a must. Specifically, experience in eukaryotic expression system (mammalian cells and/or insect cells) will be greatly appreciated.</span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">- Experience in cryoEM is desirable.<span class="gmail-Apple-converted-space"> </span></span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">- Previous experience working with GPCRs will be appreciated but isn’t essential.</span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">- The ability to work collaboratively and be a team player is essential. The candidate should also be able to work independently and efficiently.</span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">- Good oral and written skills in English is also essential as all lab business and meetings are conducted in English. French speaking is not required.</span></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">Our team, led by Philippe Rondard/ Jean-Philippe Pin, is made up of 10+ researchers, postdocs, engineers and PhD students of different nationalities forming a friendly and dynamic group. Our work mainly focuses on the study of Class C GPCRs using innovative pharmacological tools such as nanobodies and photoactivable ligands, as well as FRET to analyze GPCRs function and conformations at the molecular and single molecule level (collaboration with E Margeat, on campus). <br>The advertised position is funded by an ANR grant coordinated by Dr Anaïs Menny, who will be your direct supervisor, dedicated to expanding our research themes to single particle cryoEM and other biophysical approaches. The scientific environment at the institute also provides ample interaction possibilities and support from other teams studying GPCRs, ranging from neuropharmacology to cryoEM and crystallography.<span class="gmail-Apple-converted-space"> </span></span></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">The city of Montpellier is particularly attractive with its very large research community, dynamic cultural life, lots of bars and restaurants, affordable housing and sun all year round. It is located 20 min from the Mediterranean sea and is surrounded by vineyards<span class="gmail-Apple-converted-space"> </span>and the hilly Cevennes.</span></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none"><b>To apply, follow this link</b>: <a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__bit.ly_3HiXA9q&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TBAC-IrVW6o6oVWyU6fc2pR2u756MqEOrGtVi9PSGBvTPn10Ukqij3sTRvDqjrh4&s=V__mDDbdRTCFPjbfrqunljZhcR5fyNalc74rnpq49PI&e="><span class="gmail-s2" style="font-kerning:none">https://bit.ly/3HiXA9q</span></a></span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none"><br>
The candidate should provide a CV, a short cover letter describing their scientific interests, and name and contact details of two references.</span></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none"><br>Please contact Anaïs Menny for any informal inquiries about the position: <a href="mailto:anais.menny@igf.cnrs.fr">anais.menny@igf.cnrs.fr</a></span></p>
<p class="gmail-p3" style="margin:0px 0px 1px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none"><br></span></p><p class="gmail-p3" style="margin:0px 0px 1px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none"><br></span></p><p class="gmail-p3" style="margin:0px 0px 1px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">Relevant recent publications:</span></p>
<p class="gmail-p4" style="margin:0px 0px 3px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state. Cao AM*, Quast RB*, Fatemi F, Rondard P, Pin JP, Margeat E. Nat Commun 2021. doi:10.1038/s41467-021-25620-5</span></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">Illuminating the allosteric modulation of the calcium-sensing receptor. Liu H, Yi P, Zhao W, Wu Y,<span class="gmail-Apple-converted-space">  </span>Acher F, Pin JP, Liu J, Rondard P. PNAS September 1, 2020 117 (35) 21711-21722</span></p>
<p class="gmail-p5" style="margin:0px 0px 1px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p4" style="margin:0px 0px 3px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">Moreno Delgado D, Moller TC, Ster J, Giraldo J, Maurel D, Rovira X, Scholler P, Zwier JM, Perroy J, Durroux T, Trinquet E, Prézeau L, Rondard P and Pin J-P (2017) Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells. Elife <i>6</i>: e25233.</span></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">Structural basis for how sMAC is packaged for clearance. Menny A*, Lukassen MV*, Couves EC, Franc V, Heck AJR, Bubeck D. Nat Commun, 2021. doi: 10.1038/s41467-021-26366-w</span></p>
<p class="gmail-p2" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial;min-height:14px"><span class="gmail-s1" style="font-kerning:none"></span><br></p>
<p class="gmail-p1" style="margin:0px;text-align:justify;font-variant-numeric:normal;font-variant-east-asian:normal;font-stretch:normal;line-height:normal;font-family:Arial"><span class="gmail-s1" style="font-kerning:none">CryoEM reveals how the complement membrane attack complex ruptures lipid bilayers. Menny A, Serna M, Boyd CM, Gardner S, Joseph AP, Morgan BP, Topf M, Brooks NJ, Bubeck D. Nat Commun, 2018. doi: 10.1038/s41467-018-07653-5</span></p></div><div><br></div>-- <br><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature">Anaïs Menny<br></div></div>