[3dem] 3dem Digest, Vol 166, Issue 21

[Cazey, Cornelia]

Hi Ariel,

We used to clean our side entry holders in a Fischione 1020 Plasma cleaner that had an adapter for the specific system you use the holder in (for us FEI, but they were made for use in Jeol scopes, too). This kept them quite clean. 

I have not yet used a plasma cleaner on that much contamination (it looks like verdigris to me, is the pump ok? It usually happens in humidity), but I know for example for archaeological metal samples, plasma cleaning is often used to remove centuries old tarnish...) If you have a Material Science lab nearby, it may be worthwhile having a chat with them (about the removal as well as the availability of a suitable plasma cleaner, Fischione 1020 may only be one option).

Hope this helps,

Cornelia

Centre for Structural Systems Biology (CSSB)
c/o DESY, Building 15
Notkestr. 85
22607 Hamburg
Germany

phone: +49-(0)40-8998-87706
email: cornelia.cazey at cssb-hamburg.de

"If you don't know it's impossible, it's easier to do" -Neil Gaiman

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Sent: Tuesday, 15 June, 2021 16:21:16
Subject: 3dem Digest, Vol 166, Issue 21

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Today's Topics:

   1. rusted cryo holder 626 (Ariel Talavera)


----------------------------------------------------------------------

Message: 1
Date: Tue, 15 Jun 2021 16:21:06 +0200
From: Ariel Talavera <ariel.talavera at ulb.be>
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] rusted cryo holder 626
Message-ID: <cceac28c-bba6-614e-6d5f-a0ba828ab684 at ulb.be>
Content-Type: text/plain; charset="utf-8"; Format="flowed"

Dear all,

After an overnight bake out of the Gatan cryo holder 626 in a Gatan 
pumping station, the tip of the holder got completely rusted (attached 
photo). I read a mail (of about 5 years ago) in this mailing list 
talking about the same issue but I could not find a solution for it.
I would appreciate a lot if anyone has any suggestion, or procedure, to 
clean (if possible) the holder and remove all this rust. Should I send 
it to Gatan for them to clean it? Do you know if there is any other 
company giving this kind maintenance service for the cryo holders?

Thanks a lot advance for help
Best regards,
Ariel--

Ariel TALAVERA PEREZ, PhD
Center for Microscopy and Molecular Imaging (CMMI)
Laboratory of Microscopy
Universit? Libre de Bruxelles
Rue Adrienne Bolland, 8
B-6041 Gosselies, Belgium
Phone +32 (0)26509866


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End of 3dem Digest, Vol 166, Issue 21
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