[3dem] Per particle -ctf refine only with image name

[Jay Rai]

Hi Takanori,
Finally I am able to track down the micrograph name! So the Issue is solved.

But out of curiosity, I  I did try your way also and it works as a charm.
Thank for your suggestion.

With kind regards,
Jay
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Takanori Nakane <tnakane at mrc-lmb.cam.ac.uk>
Sent: Friday, October 2, 2020 2:36:36 AM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Per particle -ctf refine only with image name

Hi,

You don't need original micrograph MRC files but you have to
assign reasonable names to particles.

Some algorithms work per micrograph, even when you are refining
per particle. If you assign thousands of particles into one "fake"
micrograph, it will run out of memory.

If you no longer know which particle came from which image,
assign dummy micrograph names every 100 or so particles.

Best regards,

Takanori Nakane

On 2020/10/02 5:46, Jay Rai wrote:
> Hi folks
> Did anyone try relion ctf refinements with only stack? I mean no micrograph but only stacks( image name).
> I am trying to use perparticle ctf refinement in relion only from the stack, no micrograph?
> Is it duable?
> I even create dummy.mrc as the micrograph name as relion ctf refinement won’t start without micrograph name!
> I always ran out of memory even with 10000 particles.  My box is 512.
> I ran the refinement with stack and I am in 3A. I believe ctf refinement will certainly improve the resolution.
> Any help is appreciated!
> Thank you in advance
>
> With kind regards,
> Jay
>
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