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<div dir="ltr">Hi Takanori,</div>
<div dir="ltr"><span style="font-size: inherit;">Finally I am able to track down the micrograph name! So the Issue is solved. </span></div>
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<div dir="ltr">But out of curiosity, I I did try your way also and it works as a charm.</div>
<div dir="ltr">Thank for your suggestion.</div>
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<div style="direction: ltr">With kind regards,</div>
<div style="direction: ltr">Jay</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Takanori Nakane <tnakane@mrc-lmb.cam.ac.uk><br>
<b>Sent:</b> Friday, October 2, 2020 2:36:36 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> Re: [3dem] Per particle -ctf refine only with image name</font>
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<div class="PlainText">Hi,<br>
<br>
You don't need original micrograph MRC files but you have to<br>
assign reasonable names to particles.<br>
<br>
Some algorithms work per micrograph, even when you are refining<br>
per particle. If you assign thousands of particles into one "fake"<br>
micrograph, it will run out of memory.<br>
<br>
If you no longer know which particle came from which image,<br>
assign dummy micrograph names every 100 or so particles.<br>
<br>
Best regards,<br>
<br>
Takanori Nakane<br>
<br>
On 2020/10/02 5:46, Jay Rai wrote:<br>
> Hi folks<br>
> Did anyone try relion ctf refinements with only stack? I mean no micrograph but only stacks( image name).<br>
> I am trying to use perparticle ctf refinement in relion only from the stack, no micrograph?<br>
> Is it duable?<br>
> I even create dummy.mrc as the micrograph name as relion ctf refinement won’t start without micrograph name!<br>
> I always ran out of memory even with 10000 particles. My box is 512.<br>
> I ran the refinement with stack and I am in 3A. I believe ctf refinement will certainly improve the resolution.<br>
> Any help is appreciated!<br>
> Thank you in advance<br>
> <br>
> With kind regards,<br>
> Jay<br>
> <br>
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