[3dem] Hexamer dissociate while freezing Cryo-EM grids

[Farzad]

Dear All,

The other thing that should be considered is the low pH of UA staining
agent. This may affect the structure.

BR

Ferrie



On Thu, Jun 4, 2020, 22:28 Davide <dafloris at biophys.mpg.de> wrote:

> Hi Umar
>
> you can find very useful advice in our publication:
>
> *Protein denaturation at the air-water interface and how to prevent it*
>
> https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!WI2T8011J4hHlUPmqOlNq7gdeOBHNDC41J9XoHtC6PPIOAHHAjz0HU65M1ytR9W2YA$ 
> <https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!VfLdm8jk1oLaorfhmBpHU8SgDDDqoBFeugRuH6bNfz5IOsbAbVHiBNK7SqXAfdlwNw$>
>
> Best regards,
>
>
> Davide
> _________________________________________________
>
> Davide Floris
> PhD student
> Max Planck Institute of Biophysics
> Structural Biology Department
> Max-von-Laue Straße 3
> 60438 Frankfurt am Main
> Germany
>
> (L2.090): +49-69-6303-3037
> (B2.205): +49-69-6303-3049
>
> On 4. Jun 2020, at 21:24, Umar Farook <umarfarook12 at gmail.com> wrote:
>
> Dear All,
>
> I would like to ask the community for suggestions on how to overcome the
> issue of hexamer dissociating while freezing Cryo-EM grids, the negative
> staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the
> sample seems to fall apart at 1.5 mg/ml in Cryo freezing, please advise,
> thank you.
>
> Best Regards,
> Umar
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