<div dir="auto">Dear All,<div dir="auto"><br></div><div dir="auto">The other thing that should be considered is the low pH of UA staining agent. This may affect the structure.</div><div dir="auto"><br></div><div dir="auto">BR</div><div dir="auto"><br></div><div dir="auto">Ferrie</div><div dir="auto"><br></div><div dir="auto"><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Jun 4, 2020, 22:28 Davide <<a href="mailto:dafloris@biophys.mpg.de">dafloris@biophys.mpg.de</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div style="word-wrap:break-word;line-break:after-white-space">Hi Umar<div><br></div><span>you can find very useful advice in our publication:<br></span><span><br></span><span><b>Protein denaturation at the air-water interface and how to prevent it</b><br><br></span><a href="https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!VfLdm8jk1oLaorfhmBpHU8SgDDDqoBFeugRuH6bNfz5IOsbAbVHiBNK7SqXAfdlwNw$" target="_blank" rel="noreferrer">https://elifesciences.org/articles/42747</a><div><span><br></span></div><div><span>Best regards,</span></div><div><span><br></span></div><div><span><br></span></div><div><span>Davide<br></span><span><div>_________________________________________________<br><br>Davide Floris<br>PhD student<br>Max Planck Institute of Biophysics<br>Structural Biology Department<br>Max-von-Laue Straße 3<br>60438 Frankfurt am Main<br>Germany<br><br>(L2.090): +49-69-6303-3037<br>(B2.205): +49-69-6303-3049<br></div><br><blockquote type="cite">On 4. Jun 2020, at 21:24, Umar Farook <<a href="mailto:umarfarook12@gmail.com" target="_blank" rel="noreferrer">umarfarook12@gmail.com</a>> wrote:<br><br>Dear All,<br><br>I would like to ask the community for suggestions on how to overcome the issue of hexamer dissociating while freezing Cryo-EM grids, the negative staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the sample seems to fall apart at 1.5 mg/ml in Cryo freezing, please advise, thank you.<br><br>Best Regards,<br>Umar<br>_______________________________________________<br>3dem mailing list<br><a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" rel="noreferrer">3dem@ncmir.ucsd.edu</a><br><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" target="_blank" rel="noreferrer">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br></blockquote><br></span></div></div>_______________________________________________<br>
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