[3dem] Hexamer dissociate while freezing Cryo-EM grids

[Davide]

Hi Umar

you can find very useful advice in our publication:

Protein denaturation at the air-water interface and how to prevent it

https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!VfLdm8jk1oLaorfhmBpHU8SgDDDqoBFeugRuH6bNfz5IOsbAbVHiBNK7SqXAfdlwNw$  <https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!VfLdm8jk1oLaorfhmBpHU8SgDDDqoBFeugRuH6bNfz5IOsbAbVHiBNK7SqXAfdlwNw$ >

Best regards,


Davide
_________________________________________________

Davide Floris
PhD student
Max Planck Institute of Biophysics
Structural Biology Department
Max-von-Laue Straße 3
60438 Frankfurt am Main
Germany

(L2.090): +49-69-6303-3037
(B2.205): +49-69-6303-3049

> On 4. Jun 2020, at 21:24, Umar Farook <umarfarook12 at gmail.com> wrote:
> 
> Dear All,
> 
> I would like to ask the community for suggestions on how to overcome the issue of hexamer dissociating while freezing Cryo-EM grids, the negative staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the sample seems to fall apart at 1.5 mg/ml in Cryo freezing, please advise, thank you.
> 
> Best Regards,
> Umar
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