
<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Hi Umar<div class=""><br class=""></div><span class="">you can find very useful advice in our publication:<br class=""></span><span class=""><br class=""></span><span class=""><b class="">Protein denaturation at the air-water interface and how to prevent it</b><br class=""><br class=""></span><a href="https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!VfLdm8jk1oLaorfhmBpHU8SgDDDqoBFeugRuH6bNfz5IOsbAbVHiBNK7SqXAfdlwNw$" class="">https://elifesciences.org/articles/42747</a><div class=""><span class=""><br class=""></span></div><div class=""><span class="">Best regards,</span></div><div class=""><span class=""><br class=""></span></div><div class=""><span class=""><br class=""></span></div><div class=""><span class="">Davide<br class=""></span><span class=""><div class="">_________________________________________________<br class=""><br class="">Davide Floris<br class="">PhD student<br class="">Max Planck Institute of Biophysics<br class="">Structural Biology Department<br class="">Max-von-Laue Straße 3<br class="">60438 Frankfurt am Main<br class="">Germany<br class=""><br class="">(L2.090): +49-69-6303-3037<br class="">(B2.205): +49-69-6303-3049<br class=""></div><br class=""><blockquote type="cite" class="">On 4. Jun 2020, at 21:24, Umar Farook <<a href="mailto:umarfarook12@gmail.com" class="">umarfarook12@gmail.com</a>> wrote:<br class=""><br class="">Dear All,<br class=""><br class="">I would like to ask the community for suggestions on how to overcome the issue of hexamer dissociating while freezing Cryo-EM grids, the negative staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the sample seems to fall apart at 1.5 mg/ml in Cryo freezing, please advise, thank you.<br class=""><br class="">Best Regards,<br class="">Umar<br class="">_______________________________________________<br class="">3dem mailing list<br class=""><a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br class=""></blockquote><br class=""></span></div></body></html>