[3dem] Hexamer dissociate while freezing Cryo-EM grids

[Jay Rai]

Your sample might destroy by air-water interference.
Remedy- try using detergent.
Jay

With kind regards,
Jay
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Umar Farook <umarfarook12 at gmail.com>
Sent: Thursday, June 4, 2020 3:24:44 PM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: [3dem] Hexamer dissociate while freezing Cryo-EM grids

Dear All,

I would like to ask the community for suggestions on how to overcome the issue of hexamer dissociating while freezing Cryo-EM grids, the negative staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the sample seems to fall apart at 1.5 mg/ml in Cryo freezing, please advise, thank you.

Best Regards,
Umar
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