[3dem] Hexamer dissociate while freezing Cryo-EM grids

[Umar Farook]

Dear All,

I would like to ask the community for suggestions on how to overcome the
issue of hexamer dissociating while freezing Cryo-EM grids, the negative
staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the
sample seems to fall apart at 1.5 mg/ml in Cryo freezing, please advise,
thank you.

Best Regards,
Umar
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