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<div style="direction: ltr;">Your sample might destroy by air-water interference.
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<div style="direction: ltr;">Remedy- try using detergent.</div>
<div style="direction: ltr;">Jay</div>
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<div style="direction: ltr;">With kind regards,</div>
<div style="direction: ltr;">Jay</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Umar Farook <umarfarook12@gmail.com><br>
<b>Sent:</b> Thursday, June 4, 2020 3:24:44 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] Hexamer dissociate while freezing Cryo-EM grids</font>
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<div dir="auto">Dear All,
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<div dir="auto">I would like to ask the community for suggestions on how to overcome the issue of hexamer dissociating while freezing Cryo-EM grids, the negative staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the sample seems to fall apart
at 1.5 mg/ml in Cryo freezing, please advise, thank you.</div>
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<div dir="auto">Best Regards,</div>
<div dir="auto">Umar</div>
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