[3dem] Ethane Condensation Dewar

[John Rubinstein]

Hi Joshua,

For both our modified Mark III Vitrobot and our high-speed ultrasonic grid freezing device (https://journals.iucr.org/d/issues/2019/12/00/ih5001/) we use an aluminum cryogen container within an insulating container.
In order to avoid ethane freezing near liquid nitrogen temperature we mix it with propane (from propane torch cylinders) according to Bill Tivol’s method (https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/an-improved-cryogen-for-plunge-freezing/C791DB9759F82173E83309E1F8935101)

The aluminum part for our ultrasonic device was designed to fit into the small styrofoam boxes used by AbCam.
The design files are here (let me know if you need a different format):
https://github.com/johnrubinstein/SIOcontrol/tree/master/CAD_files
The part can be made by CNC milling, which is available as a web service from several companies (or you may be able to do it in your machine shop).

Best,
John


—
John Rubinstein
Molecular Medicine Program
The Hospital for Sick Children
686 Bay Street, Rm. 20-9705
M5G 0A4
(+001) 416-813-7255
www.rubinsteinlab.org<http://www.rubinsteinlab.org>



On Jan 21, 2020, at 8:22 PM, Joshua Denson <josh.denson2 at gmail.com<mailto:josh.denson2 at gmail.com>> wrote:

Hello 3DEM community,

Our lab is currently working to set-up a manual plunge freezing device as a cost effective alternative to grid preparation using the Vitrobot. We are currently working off of a design we found on Github out of Purdue University (referenced here a few times) that seems reasonable for our machine shop to manufacture.

The one thing we are having difficulty finding is some kind of a stand-alone ethane condensation dewar. A temperature regulated device would be nice, but something similar to what is used on the Vitrobot would also work well.

I was wondering if anyone would be able to direct me to a place that I could purchase one, or if there are any designs out there for getting one made, that would also be great.

Best,
Joshua
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