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Hi Joshua,
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<div class="">For both our modified Mark III Vitrobot and our high-speed ultrasonic grid freezing device (<a href="https://journals.iucr.org/d/issues/2019/12/00/ih5001/" class="">https://journals.iucr.org/d/issues/2019/12/00/ih5001/</a>) we use an aluminum
cryogen container within an insulating container.</div>
<div class="">In order to avoid ethane freezing near liquid nitrogen temperature we mix it with propane (from propane torch cylinders) according to Bill Tivol’s method (<a href="https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/an-improved-cryogen-for-plunge-freezing/C791DB9759F82173E83309E1F8935101" class="">https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/an-improved-cryogen-for-plunge-freezing/C791DB9759F82173E83309E1F8935101</a>)</div>
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<div class="">The aluminum part for our ultrasonic device was designed to fit into the small styrofoam boxes used by AbCam.</div>
<div class="">The design files are here (let me know if you need a different format):</div>
<div class=""><a href="https://github.com/johnrubinstein/SIOcontrol/tree/master/CAD_files" class="">https://github.com/johnrubinstein/SIOcontrol/tree/master/CAD_files</a></div>
<div class="">The part can be made by CNC milling, which is available as a web service from several companies (or you may be able to do it in your machine shop).</div>
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<div class="">Best,</div>
<div class="">John</div>
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<div>— </div>
<div>John Rubinstein</div>
<div>Molecular Medicine Program</div>
<div>The Hospital for Sick Children</div>
<div>686 Bay Street, Rm. 20-9705</div>
<div>M5G 0A4</div>
<div>(+001) 416-813-7255</div>
<div><a href="http://www.rubinsteinlab.org" class="">www.rubinsteinlab.org</a></div>
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<div class="">On Jan 21, 2020, at 8:22 PM, Joshua Denson <<a href="mailto:josh.denson2@gmail.com" class="">josh.denson2@gmail.com</a>> wrote:</div>
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<div dir="ltr" class="">Hello 3DEM community,
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<div class="">Our lab is currently working to set-up a manual plunge freezing device as a cost effective alternative to grid preparation using the Vitrobot. We are currently working off of a design we found on Github out of Purdue University (referenced here
a few times) that seems reasonable for our machine shop to manufacture. </div>
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<div class="">The one thing we are having difficulty finding is some kind of a stand-alone ethane condensation dewar. A temperature regulated device would be nice, but something similar to what is used on the Vitrobot would also work well. </div>
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<div class="">I was wondering if anyone would be able to direct me to a place that I could purchase one, or if there are any designs out there for getting one made, that would also be great. </div>
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<div class="">Best,</div>
<div class="">Joshua</div>
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