[3dem] A Request for Micrograph with multiple 3d particle or particle conformations

[Takanori Nakane]

Hi,

How different should it be?

Different species:
EMPIAR-10251: mixture of 20S proteasome and TMV
EMPIAR 10289-10291: innexin hemichannel but contains dimeric full 
channels as well

Conformational difference:
EMPIAR-10180: spliceosome with flexibility
EMPIAR-10160: spliceosome, even more flexibility

And there are several ribosome datasets as well.

Best regards,

Takanori Nakane

On 2020/04/20 19:03, rupesh wrote:
> Dear all,
> 
> I have been working on 3D reconstructions from 2d projections obtained 
> by Cryo Electron Microscopy, specially dealing with the case when there 
> are multiple conformations of the particle in the projections obtained 
> and hence multiple 3D structures to be generated.
> 
> I have obtained good classifications results in case of projections 
> obtained for different particles from different micrographs, but to test 
> the classifier in its full capacity, I need a micrograph which has 2 or 
> more types of picked particles, and hence 2 or more 3D structure lying 
> in the set of projections that can be obtained.
> 
> Any help or pointers to correct resources will be highly appreciated.
> 
> Regards
> Rupesh
> 4th Year UnderGrad
> Computer Science
> IIT Bombay
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