[3dem] 3dem Digest, Vol 152, Issue 6

[Cynthia Lee Page]

Hi Huy, Thank you for your response, I should have made myself more clear with this request. I *should” have done the 3D Classification in Relion 3.0, you are correct :(. Before I go back and do this, I was hoping someone had developed a solution in this case (wishful thinking :)).

Thanks again,

Cynthia

> On Apr 4, 2020, at 1:00 PM, 3dem-request at ncmir.ucsd.edu wrote:
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>   1. Re: [ccpem] MiRP, Error more columns than column names (Huy Bui)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Sat, 4 Apr 2020 12:06:09 -0400
> From: Huy Bui <huybk81 at gmail.com>
> To: 3dem at ncmir.ucsd.edu
> Subject: Re: [3dem] [ccpem] MiRP, Error more columns than column names
> Message-ID:
> 	<CADBiWEaZs7TYNBmvQik_7N1uN32j=Y-7S9c2SNvs=8-fG-dBMQ at mail.gmail.com>
> Content-Type: text/plain; charset="utf-8"
> 
> Hi Cynthia,
> 
> That's because of Relion 3.1 using the data_optics block in the star
> file. Almost all of the scripts for MiRP will fail to process Relion 3.1
> properly due to different column orders.
> 
> I suggest that you should use relion 3.0 instead of relion 3.1 for the
> MiRP.
> 
> Best,
> Huy
> 
> On Sat, Apr 4, 2020 at 11:54 AM Cynthia Page <cynthia.page at colorado.edu>
> wrote:
> 
>> Hi,
>> 
>> Is anyone using the Moores Lab/MiRP procedure in conjunction with Relion
>> 3.1, that can offer advise? I am getting an error in step 9 of the readme,
>> Unifying 3D Classes.
>> 
>> After running reformat.pl on the run_it001_data.star file and using the
>> output in R,
>> and after the first line of R commands:
>> 
>> x= read.table (?run_it001_data_corrected00.star?, header= T)
>> 
>> the program returns the error, more columns than column names.
>> 
>> Thanks for any help!
>> 
>> Cynthia
>> 
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