[3dem] [ccpem] MiRP, Error more columns than column names

Huy Bui huybk81 at gmail.com
Sat Apr 4 09:06:09 PDT 2020


Hi Cynthia,

That's because of Relion 3.1 using the data_optics block in the star
file. Almost all of the scripts for MiRP will fail to process Relion 3.1
properly due to different column orders.

I suggest that you should use relion 3.0 instead of relion 3.1 for the
MiRP.

Best,
Huy

On Sat, Apr 4, 2020 at 11:54 AM Cynthia Page <cynthia.page at colorado.edu>
wrote:

> Hi,
>
> Is anyone using the Moores Lab/MiRP procedure in conjunction with Relion
> 3.1, that can offer advise? I am getting an error in step 9 of the readme,
> Unifying 3D Classes.
>
> After running reformat.pl on the run_it001_data.star file and using the
> output in R,
> and after the first line of R commands:
>
> x= read.table (“run_it001_data_corrected00.star”, header= T)
>
> the program returns the error, more columns than column names.
>
> Thanks for any help!
>
> Cynthia
>
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