[3dem] Gatan Rio camera experience

[Tobias Furstenhaupt]

Dear Andrei,

we don’t have a Rio so I cannot contribute anything useful to this
discussion.
But just to clarify: Gatan already exchanged your hardware (ie the
controller)? And they are 'confused' about your issues? Meaning the
all-too-often answer "Oh, you are the first with those issues"?

cheers
Tobias
 

-----------------------------------
Tobias Fürstenhaupt, PhD
head of Electron Microscopy
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstrasse 108
01307 Dresden, Germany

phone: (+49) (0)351 210-2690



-----Original Message-----
From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Andrei
Moiseenko
Sent: 27 September 2019 20:10
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Gatan Rio camera experience

Dear all,

we got a Gatan Rio 9 camera, which unfortunately came with a plenty of
bugs. I never touched this type of a camera before, so I wonder if anyone
has experienced such Rio behavior:

1)      The “New Gain Reference” task asks user to adjust beam intensity
only once, however it should ask twice, as written in user manual. First
time for a High-Gain ADC (250-350 counts), and second time for a Low-Gain
ADC (2000-3333 counts).

2)      “Frames to average” gain reference option is only applied to a
Bin1 high-gain reference (.x1.m0.g1), but not to all other ref images

3)      Dark pixels are vastly present over each gain normalized image
taken with high beam intensity. The intensities of these pixels are twice
lower than they should be, and those pixels are very likely to appear due
to a wrong low-gain reference, which is taken at insufficient intensity
(see above). Despite the fact that locations of those pixels looks like
noise, they produce the FFT peaks exactly the same as honeycomb structure.
Moreover, dark pixel’s coordinates remain the same unless you take a new
gain reference.

4)      Some frames coming from a camera are randomly shifted 1 px
horizontally, which leads to a wrong gain ref correction, so honeycomb
structure becomes visible over the whole summed stack.



Gatan is completely confused with these issues, because even changing the
camera hardware did not solve any of them. From my point of view, at least
some of these issues are highly likely to be software bugs. So I ask for
any experience of using Rio, especially about 1) and 4). Thanks!



Best regards
Andrey Moiseenko

Electron Microscopy Lab @ Biology Department Moscow State University
postmoiseenko at gmail.com <mailto:postmoiseenko at gmail.com>
skype: callmoiseenko