[3dem] Gatan Rio camera experience

[Andrei Moiseenko]

Dear all,

we got a Gatan Rio 9 camera, which unfortunately came with a plenty of bugs.
I never touched this type of a camera before, so I wonder if anyone has
experienced such Rio behavior:

1)      The "New Gain Reference" task asks user to adjust beam intensity
only once, however it should ask twice, as written in user manual. First
time for a High-Gain ADC (250-350 counts), and second time for a Low-Gain
ADC (2000-3333 counts). 

2)      "Frames to average" gain reference option is only applied to a Bin1
high-gain reference (.x1.m0.g1), but not to all other ref images

3)      Dark pixels are vastly present over each gain normalized image taken
with high beam intensity. The intensities of these pixels are twice lower
than they should be, and those pixels are very likely to appear due to a
wrong low-gain reference, which is taken at insufficient intensity (see
above). Despite the fact that locations of those pixels looks like noise,
they produce the FFT peaks exactly the same as honeycomb structure.
Moreover, dark pixel's coordinates remain the same unless you take a new
gain reference.

4)      Some frames coming from a camera are randomly shifted 1 px
horizontally, which leads to a wrong gain ref correction, so honeycomb
structure becomes visible over the whole summed stack. 

 

Gatan is completely confused with these issues, because even changing the
camera hardware did not solve any of them. From my point of view, at least
some of these issues are highly likely to be software bugs. So I ask for any
experience of using Rio, especially about 1) and 4). Thanks!

 

Best regards
Andrey Moiseenko

Electron Microscopy Lab @ Biology Department
Moscow State University
 <mailto:postmoiseenko at gmail.com> postmoiseenko at gmail.com 
skype: callmoiseenko

 

 

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20190927/5762a408/attachment.html>