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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Dear all,<o:p></o:p></p><p class=MsoNormal>we got a Gatan Rio 9 camera, which unfortunately came with a plenty of bugs. I never touched this type of a camera before, so I wonder if anyone has experienced such Rio behavior:<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>1)<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>The “New Gain Reference” task asks user to adjust beam intensity <i>only once</i>, however it should ask twice, as written in user manual. First time for a High-Gain ADC (250-350 counts), and second time for a Low-Gain ADC (2000-3333 counts). <o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>2)<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>“Frames to average” gain reference option is only applied to a Bin1 high-gain reference (.x1.m0.g1), but not to all other ref images<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>3)<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Dark pixels are vastly present over each gain normalized image taken with high beam intensity. The intensities of these pixels are twice lower than they should be, and those pixels are very likely to appear due to a wrong low-gain reference, which is taken at insufficient intensity (see above). Despite the fact that locations of those pixels looks like noise, they produce the FFT peaks exactly the same as honeycomb structure. Moreover, dark pixel’s coordinates remain the same unless you take a new gain reference.<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>4)<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Some frames coming from a camera are randomly shifted 1 px horizontally, which leads to a wrong gain ref correction, so honeycomb structure becomes visible over the whole summed stack. <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Gatan is completely confused with these issues, because even changing the camera hardware did not solve any of them. >From my point of view, at least some of these issues are highly likely to be software bugs. So I ask for any experience of using Rio, especially about 1) and 4). Thanks!<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Best regards<br>Andrey Moiseenko<o:p></o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Electron Microscopy Lab @ Biology Department<br>Moscow State University<br><a href="mailto:postmoiseenko@gmail.com"><span style='color:blue'>postmoiseenko@gmail.com</span></a> <br>skype: callmoiseenko<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>