[3dem] 3D printed cryo-grid boxes

[Sue Tivol]

Hi Davide, et al.,
	Instead of using the grid box lids, one can look in the appropriate catalog to find plastic sheets that can stand low temps ~1 mm thick, ~30 cm square, find a 13 mm punch, and cut many lids from one sheet.  I had my shop make a jig in which I could stack about a dozen disks, drill center holes, and an offset hole, which became a U-shaped slot with a little Dremel work.
						Yours,
						Bill


> On Jun 10, 2019, at 10:19 PM, Eric Hanssen <ehanssen at unimelb.edu.au> wrote:
> 
> Hi Davide,
> what I do is I bring the empty grid boxes that comes with the grids we purchase (the blue kind) to our workshop and they cut the boxes and lids in little 4 grid squares, add a little screw (Teflon, but my last batch was metal and they are much better) et voila!  Cost $1 per grid box for the labour
> 
> Assoc. Prof Eric Hanssen
> Head - Advanced Microscopy Facility
> Honorary Principal Fellow – Department of Biochemistry and Molecular Biology
> 
> President Australian Microscopy and Microanalysis Society
> 
> Bio21 Molecular Science and Biotechnology Institute - 30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia
> email: ehanssen at unimelb.edu.au <mailto:ehanssen at unimelb.edu.au> | Office: +61 3 83442449 | Microscope: +61 3 83442509 
> Web: www.microscopy.unimelb.edu.au <http://www.microscopy.unimelb.edu.au/>
>>  
> 
> 
> On 11 Jun 2019, at 12:46, John Crum <jcrum12 at cox.net <mailto:jcrum12 at cox.net>> wrote:
> 
>> Davide,
>> 
>> I have modeled and printed cryo grid boxes. I have not used them, though. I decided not to pursue making and selling them because the cost was about the same as the commercially available ones (about USD 9). I'm not sure that the plastic from any 3D printer type would stand up to LN2 temperatures, over the long run. I do make and sell a manual plunge freezing workstation. You can see it on the 3D printer service "Shapeways" at:
>> 
>> https://www.shapeways.com/product/HZSNAVE4B/plungefreezeinsert-base-v5-1-3 <https://www.shapeways.com/product/HZSNAVE4B/plungefreezeinsert-base-v5-1-3>
>> 
>> Let me know if you have any questions...
>> 
>> jc
>> 
>> 
>> John Crum
>> JC Designs/California
>> 
>>> On June 10, 2019 at 2:22 AM Davide Zabeo <davide.zabeo at gu.se <mailto:davide.zabeo at gu.se>> wrote: 
>>> 
>>> 
>>> 
>>> Dear all,
>>> 
>>> 
>>> 
>>> In our lab we would like to try 3D-printing cryo-boxes to store plunge frozen cryo-grids (attached photo as reference).
>>> 
>>> 
>>> 
>>> Has anyone tried this before? Does anyone have a blueprint of it?
>>> 
>>> 
>>> 
>>> Any help is appreciated! Thank you.
>>> 
>>> 
>>> 
>>> Best wishes,
>>> 
>>> 
>>> 
>>> Davide Zabeo
>>> 
>>> PhD student
>>> 
>>> Johanna Höög Lab
>>> 
>>> Department of Chemistry and Molecular Biology
>>> 
>>> University of Gothenburg
>>> Visiting Address: Medicinaregatan 9 C, 41390 Göteborg 
>>> Postal Address: Box 462, 40530 Göteborg SWEDEN
>>> 
>>> 
>>> 
>> 
>>  
>>> 
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