<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class="">Hi Davide, et al.,</div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Instead of using the grid box lids, one can look in the appropriate catalog to find plastic sheets that can stand low temps ~1 mm thick, ~30 cm square, find a 13 mm punch, and cut many lids from one sheet. I had my shop make a jig in which I could stack about a dozen disks, drill center holes, and an offset hole, which became a U-shaped slot with a little Dremel work.</div><div style="orphans: 2; widows: 2;" class=""><span class="Apple-tab-span" style="white-space: pre;"> </span>Yours,</div><div style="orphans: 2; widows: 2;" class=""><span class="Apple-tab-span" style="white-space: pre;"> </span>Bill</div><div class=""><br class=""></div><div><br class=""><blockquote type="cite" class=""><div class="">On Jun 10, 2019, at 10:19 PM, Eric Hanssen <<a href="mailto:ehanssen@unimelb.edu.au" class="">ehanssen@unimelb.edu.au</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">
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Hi Davide,
<div class="">what I do is I bring the empty grid boxes that comes with the grids we purchase (the blue kind) to our workshop and they cut the boxes and lids in little 4 grid squares, add a little screw (Teflon, but my last batch was metal and they are much better)
et voila! Cost $1 per grid box for the labour<br class="">
<br class="">
<div dir="ltr" class="">Assoc. Prof Eric Hanssen
<div class=""><b style="font-size: 13pt; background-color: rgba(255, 255, 255, 0);" class="">Head - Advanced Microscopy Facility</b></div>
<div class=""><b style="font-size: 13pt; background-color: rgba(255, 255, 255, 0);" class="">Honorary Principal Fellow – Department of Biochemistry and Molecular Biology</b></div>
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</b></div>
<div class=""><b style="background-color: rgba(255, 255, 255, 0);" class="">President Australian Microscopy and Microanalysis Society</b></div>
<div class=""><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class=""><br class="">
</span></div>
<div class=""><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class="">Bio21 Molecular Science and Biotechnology Institute - </span><span style="font-size: 13pt; background-color: rgba(255, 255, 255, 0);" class="">30 Flemington Road - The University of Melbourne
- Victoria 3010 - Australia</span></div>
<div class=""><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class="">email:</span><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class=""> </span><a href="mailto:ehanssen@unimelb.edu.au" style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class="">ehanssen@unimelb.edu.au</a><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class=""> </span><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class="">|
Office: +61 3 83442449 | Microscope: +61 3 83442509</span><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;" class=""> </span></div>
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On 11 Jun 2019, at 12:46, John Crum <<a href="mailto:jcrum12@cox.net" class="">jcrum12@cox.net</a>> wrote:<br class="">
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<div dir="ltr" class="">Davide,<br class="">
<br class="">
I have modeled and printed cryo grid boxes. I have not used them, though. I decided not to pursue making and selling them because the cost was about the same as the commercially available ones (about USD 9). I'm not sure that the plastic from any 3D printer
type would stand up to LN2 temperatures, over the long run. I do make and sell a manual plunge freezing workstation. You can see it on the 3D printer service "Shapeways" at:<br class="">
<br class="">
<a href="https://www.shapeways.com/product/HZSNAVE4B/plungefreezeinsert-base-v5-1-3" class="">https://www.shapeways.com/product/HZSNAVE4B/plungefreezeinsert-base-v5-1-3</a><br class="">
<br class="">
Let me know if you have any questions...<br class="">
<br class="">
jc<br class="">
<br class="">
<br class="">
John Crum<br class="">
JC Designs/California<br class="">
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<blockquote type="cite" class="">On June 10, 2019 at 2:22 AM Davide Zabeo <<a href="mailto:davide.zabeo@gu.se" class="">davide.zabeo@gu.se</a>> wrote:
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<div id="divtagdefaultwrapper" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;" dir="ltr" class=""><div class=""><br class="webkit-block-placeholder"></div>
<div id="divtagdefaultwrapper" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;" dir="ltr" class=""><div class=""><br class="webkit-block-placeholder"></div>
<div id="divtagdefaultwrapper" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;" dir="ltr" class=""><p class="">Dear all,</p><p class=""><br class="">
</p><p class="">In our lab we would like to try 3D-printing cryo-boxes to store plunge frozen cryo-grids (attached photo as reference).</p><p class=""><br class="">
</p><p class="">Has anyone tried this before? Does anyone have a blueprint of it?</p><p class=""><br class="">
</p><p class="">Any help is appreciated! Thank you.</p><p class=""><br class="">
</p><p class="">Best wishes,</p><p class=""><br class="">
</p>
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