[3dem] 3D printed cryo-grid boxes

[Zhijie Li]

Hi David,


Here are some of my designs:


https://github.com/zhijie-li/cyoEMtools


The cryo grid box is of a square shape. So I also put a vitrobot stand for the square boxes.  The stand will contract quite a bit in LN2 so please test with your LN2 container first.

I also suggest using a 0.3 mm nozzle for printing the cryo grid boxes to make the corners as sharp as possible.


Zhijie


________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Davide Zabeo <davide.zabeo at gu.se>
Sent: Monday, June 10, 2019 5:22 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] 3D printed cryo-grid boxes


Dear all,


In our lab we would like to try 3D-printing cryo-boxes to store plunge frozen cryo-grids (attached photo as reference).


Has anyone tried this before? Does anyone have a blueprint of it?


Any help is appreciated! Thank you.


Best wishes,


Davide Zabeo

PhD student

Johanna Höög Lab

Department of Chemistry and Molecular Biology

University of Gothenburg
Visiting Address: Medicinaregatan 9 C, 41390 Göteborg
Postal Address: Box 462, 40530 Göteborg SWEDEN
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