[3dem] Elsa cryo holder

Wed Jun 12 10:09:37 PDT 2019

Hi Anastasia,

We have been testing the same configuration and observed the same thing, BUT not always. We are still trying to get to the bottom of it but we have had some successful transfers. My current hunch is that the insulation and resulting thermic inertia of the ELSA holder, while making extra stable in the microscope column causes it to take a very, very long time to cool down initially. There could be a very slight warming up of the grid during transfer causing the strange ice pattern if not waiting long enough. We have had some success when we waited more than 1hr after starting to cool it down before transfer. Our experience is still limited though.

I would be very interested in hearing about other people’s experience.

Cheers,

Patricia

_______________________________
Patricia Grob, Ph.D.
Research Specialist
Nogales Lab
HHMI at UC Berkeley
Molecular & Cell Biology Department / QB3,
Stanley Hall #742
University of California, Berkeley
Berkeley, CA 94720-3220
Phone: (510)666-3335	Fax: (510)666-3336
E.M. suite B307: (510)666-3339
URL: cryoem.berkeley.edu
Email: pgrob at berkeley.edu

> On Jun 12, 2019, at 5:00 AM, Anastasia Gazi <anadegaz at gmail.com> wrote:
> 
> Dear all,
>  
> I would be grateful if someone of you could share some more information related to the ELSA cryoholders.
>  
> We extensively tried out the ultra-low profile (ULP) tip for 2 weeks and the results were quite controversial.
> The stability of the holder was amazing in comparison to our 626 cryoholders but there was a regular appearing of leopard/orange skin on the grids.
> We believe that this might be related to leakage at the loading station but I wonder if someone else had the same experience with its own ELSA (ULP or standard tip profile).
> 
>  
> Thank you a lot for your help,
> Anastasia  
> 
> On Sat, 6 Jan 2018 at 00:38, David Michael Belnap <David.Belnap at utah.edu <mailto:David.Belnap at utah.edu>> wrote:
> I don’t know, Benjamin.  Elsa freezes things so fast that maybe she is really making vitreous ice!  :-)
> 
> 
> 
>> On Jan 4, 2018, at 10:13 AM, Benjamin Bammes <bbammes at gmail.com <mailto:bbammes at gmail.com>> wrote:
>> 
>> While I don't know anything about the holder, I find the name "Elsa" a bit humorous. For anyone with young kids who has watched the movie Frozen, you know that Elsa does two things: (1) She turns everything she touches to solid ice, and (2) She always wants to "let it go." Both of those things are undesirable for cryo holders! :-)
>> 
>> ----
>> Benjamin Bammes
>> 
>> On Wed, Jan 3, 2018 at 9:21 PM, jamonshi <jamonshi at gmail.com <mailto:jamonshi at gmail.com>> wrote:
>> Our local sales says
>> 
>> "Gatan has not shipped any production 698 Elas holder yet. The holders won’t start shipping until March of 2018. Gatan cannot discover where they tested the holder, as that is not a complete product and the development is confidential." 
>> 
>> Cheers,
>> Jian
>> 
>> On 04/01/2018 06:36, Elizabeth Montabana wrote:
>>> Hi All
>>> 
>>> I haven't seen any responses to this, but we are also curious about if anyone has purchased or tested an Elsa Cryo transfer holder and their opinion about it - particularly, if folks have been able to get high resolution data with it. 
>>> 
>>> thanks
>>> Liz Montabana
>>> Donner Labs, LBNL
>>> 
>>> On Wed, Nov 29, 2017 at 4:54 AM, Anastasia Gazi <anadegaz at gmail.com <mailto:anadegaz at gmail.com>> wrote:
>>>  <mailto:3dem at ncmir.ucsd.edu>Hello all,
>>> 
>>> 
>>> 
>>> We are considering the purchase of an Elsa Cryo transfer holder Model 698 from Gatan. It is looking great on paper with its centrosymmetric design, extended hold time to 8h and everything but I am wondering if someone has already experience with it. Either with the standard profile holder or with the ultra-low one. 
>>> 
>>> 
>>> 
>>> Thank you for your help,
>>> 
>>> Anastasia
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> ==================================================
>>> Anastasia D. Gazi, PhD.
>>> Ultrastructural BioImaging unit,
>>> Départment de Biologie cellulaire et infection,
>>> Institut Pasteur
>>> 28, rue du Dr. Roux
>>> 75015 PARIS
>>> FRANCE
>>> 
>>> agazi at pasteur.fr <mailto:agazi at pasteur.fr>
>>> ==================================================
>>> 
>>> 
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