<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Anastasia,<div class=""><br class=""></div><div class="">We have been testing the same configuration and observed the same thing, BUT not always. We are still trying to get to the bottom of it but we have had some successful transfers. My current hunch is that the insulation and resulting thermic inertia of the ELSA holder, while making extra stable in the microscope column causes it to take a very, very long time to cool down initially. There could be a very slight warming up of the grid during transfer causing the strange ice pattern if not waiting long enough. We have had some success when we waited more than 1hr after starting to cool it down before transfer. Our experience is still limited though.</div><div class=""><br class=""></div><div class="">I would be very interested in hearing about other people’s experience.</div><div class=""><br class=""></div><div class="">Cheers,</div><div class=""><br class=""></div><div class="">Patricia</div><div class=""><br class=""><div class="">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">_______________________________<br class=""><font color="#929292" class="">Patricia Grob, Ph.D.<br class="">Research Specialist<br class="">Nogales Lab<br class="">HHMI at UC Berkeley<br class="">Molecular & Cell Biology Department / QB3,<br class="">Stanley Hall #742<br class="">University of California, Berkeley<br class="">Berkeley, CA 94720-3220<br class="">Phone: (510)666-3335<span class="Apple-tab-span" style="white-space: pre;"> </span>Fax: (510)666-3336<br class="">E.M. suite B307: (510)666-3339<br class="">URL: <a href="http://cryoem.berkeley.edu" class="">cryoem.berkeley.edu</a><br class="">Email: <a href="mailto:pgrob@berkeley.edu" class="">pgrob@berkeley.edu</a><br class=""><br class=""><br class=""></font><br class=""></div></div>
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<br class=""><div><blockquote type="cite" class=""><div class="">On Jun 12, 2019, at 5:00 AM, Anastasia Gazi <<a href="mailto:anadegaz@gmail.com" class="">anadegaz@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class=""><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Dear all, <span class=""></span></div><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:11pt;font-family:"Calibri",sans-serif"><span class=""> </span></p><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">I would be grateful if someone of you could share some more information related to the ELSA cryoholders.<span class=""></span></div><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:11pt;font-family:"Calibri",sans-serif"><span class=""> </span></p><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">We extensively tried out the ultra-low profile (ULP) tip for 2 weeks and the results were quite controversial. <span class=""></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">The stability of the holder was amazing in comparison to our 626 cryoholders but there was a regular appearing of leopard/orange skin on the grids. <span class=""></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">We believe that this might be related to leakage at the loading station but I wonder if someone else had the same experience with its own ELSA (ULP or standard tip profile).<span class=""></span></div>
<span class=""></span><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
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<span class=""></span></div><p class="MsoNormal" style="margin:0in 0in 0.0001pt;font-size:11pt;font-family:"Calibri",sans-serif"><span class=""> </span></p><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Thank you a lot for your help, <span class=""></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Anastasia <span class=""> </span><span class=""></span></div>
</div><br class=""><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Sat, 6 Jan 2018 at 00:38, David Michael Belnap <<a href="mailto:David.Belnap@utah.edu" class="">David.Belnap@utah.edu</a>> wrote:<br class=""></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
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I don’t know, Benjamin. Elsa freezes things so fast that maybe she is really making vitreous ice! :-)
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<div class="">On Jan 4, 2018, at 10:13 AM, Benjamin Bammes <<a href="mailto:bbammes@gmail.com" target="_blank" class="">bbammes@gmail.com</a>> wrote:</div>
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<div class="">
<div dir="ltr" class="">While I don't know anything about the holder, I find the name "Elsa" a bit humorous. For anyone with young kids who has watched the movie Frozen, you know that Elsa does two things: (1) She turns everything she touches to solid ice,
and (2) She always wants to "let it go." Both of those things are undesirable for cryo holders! :-)<br class="">
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<div dir="ltr" class=""><font size="2" class="">----<br class="">
<b class="">Benjamin Bammes</b></font></div>
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<div class="gmail_quote">On Wed, Jan 3, 2018 at 9:21 PM, jamonshi <span dir="ltr" class="">
<<a href="mailto:jamonshi@gmail.com" target="_blank" class="">jamonshi@gmail.com</a>></span> wrote:<br class="">
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<div bgcolor="#FFFFFF" class=""><p class="">Our local sales says</p><p class="">"<span lang="EN-US" class="">Gatan has not shipped any production 698 Elas holder yet. The holders won’t start shipping until March of 2018. Gatan cannot discover where they tested the holder, as that is not a complete product and the development
is confidential." <br class="">
</span></p>
Cheers,<br class="">
Jian
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<div class="gmail-m_-6136389263333342439m_-6433392509370616762m_2846594650987020078moz-cite-prefix">On 04/01/2018 06:36, Elizabeth Montabana wrote:<br class="">
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<div dir="ltr" class="">Hi All
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<div class="">I haven't seen any responses to this, but we are also curious about if anyone has purchased or tested an Elsa Cryo transfer holder and their opinion about it - particularly, if folks have been able to get high resolution data with it. </div>
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<div class="">thanks</div>
<div class="">Liz Montabana</div>
<div class="">Donner Labs, LBNL</div>
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<div class="gmail_quote">On Wed, Nov 29, 2017 at 4:54 AM, Anastasia Gazi <span dir="ltr" class="">
<<a href="mailto:anadegaz@gmail.com" target="_blank" class="">anadegaz@gmail.com</a>></span> wrote:<br class="">
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<div dir="ltr" class=""><a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" class=""></a><p class="MsoNormal" style="line-height:normal"><span class="">Hello all,</span></p><p class="MsoNormal" style="line-height:normal"><span class=""><br class="">
</span></p><p class="MsoNormal" style="line-height:normal"><span class="">We are considering the purchase of an Elsa Cryo transfer holder Model 698 from Gatan. It is looking great on paper with its centrosymmetric design, extended hold time to 8h and everything but I
am wondering if someone has already experience with it. Either with the standard profile holder or with the ultra-low one.
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</span></p><p class="MsoNormal" style="line-height:normal"><span class=""><br class="">
</span></p><p class="MsoNormal" style="line-height:normal"><span class="">Thank you for your help,
</span></p><p class="MsoNormal" style="line-height:normal"><span class="">Anastasia</span></p><p class="MsoNormal" style="line-height:normal"><span class=""><br class="">
</span></p><p class="MsoNormal" style="line-height:normal"><span class=""><br class="">
</span></p><p class="MsoNormal" style="line-height:normal"><span class=""><br class="">
</span></p><p class="MsoNormal" style="line-height:normal"><span class="">==================================================<br class="">
Anastasia D. Gazi, PhD.<br class="">
Ultrastructural BioImaging unit,<br class="">
Départment de Biologie cellulaire et infection,<br class="">
Institut Pasteur<br class="">
28, rue du Dr. Roux<br class="">
75015 PARIS<br class="">
FRANCE</span></p><p class="MsoNormal" style="line-height:normal"><span class=""><a href="mailto:agazi@pasteur.fr" target="_blank" class="">agazi@pasteur.fr</a></span></p><p class="MsoNormal" style="line-height:normal"><span class="">==================================================<br class="">
</span></p>
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