[3dem] Elsa cryo holder

Wed Jun 12 05:47:30 PDT 2019

Dear Anastasia,

The leakage at the loading station is not a malfunction but a design feature. We thought our leaking station was defective, but Gatan responded that the Elsa station is not supposed to be filled with nitrogen to the level where is starts to leak (if you look closely, there is a small hole through which liquid nitrogen escapes). The grid is not supposed to be covered with liquid nitrogen; it is kept cold with nitrogen vapors.  This makes me somewhat uncomfortable but the approach seems to be working fine. As for the holder’s performance, we have only used our Elsa a few times, and I cannot comment on consistency of ice quality. The hold time, however, is amazing: 11 hours confirmed.

Best regards,
Yaroslav

Yaroslav Tsybovsky, Ph.D. (Contractor)
Scientist II
Frederick National Laboratory for Cancer Research
Leidos Biomedical Research, Inc.
Post Office Box B
Frederick, Maryland 21702
Phone: 301-846-7550

From: Anastasia Gazi <anadegaz at gmail.com>
Sent: Wednesday, June 12, 2019 8:01 AM
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] Elsa cryo holder

Dear all,

I would be grateful if someone of you could share some more information related to the ELSA cryoholders.

We extensively tried out the ultra-low profile (ULP) tip for 2 weeks and the results were quite controversial.
The stability of the holder was amazing in comparison to our 626 cryoholders but there was a regular appearing of leopard/orange skin on the grids.
We believe that this might be related to leakage at the loading station but I wonder if someone else had the same experience with its own ELSA (ULP or standard tip profile).

Thank you a lot for your help,
Anastasia

On Sat, 6 Jan 2018 at 00:38, David Michael Belnap <David.Belnap at utah.edu<mailto:David.Belnap at utah.edu>> wrote:
I don’t know, Benjamin.  Elsa freezes things so fast that maybe she is really making vitreous ice!  :-)

On Jan 4, 2018, at 10:13 AM, Benjamin Bammes <bbammes at gmail.com<mailto:bbammes at gmail.com>> wrote:

While I don't know anything about the holder, I find the name "Elsa" a bit humorous. For anyone with young kids who has watched the movie Frozen, you know that Elsa does two things: (1) She turns everything she touches to solid ice, and (2) She always wants to "let it go." Both of those things are undesirable for cryo holders! :-)

----
Benjamin Bammes

On Wed, Jan 3, 2018 at 9:21 PM, jamonshi <jamonshi at gmail.com<mailto:jamonshi at gmail.com>> wrote:

Our local sales says

"Gatan has not shipped any production 698 Elas holder yet. The holders won’t start shipping until March of 2018. Gatan cannot discover where they tested the holder, as that is not a complete product and the development is confidential."
Cheers,
Jian

On 04/01/2018 06:36, Elizabeth Montabana wrote:
Hi All

I haven't seen any responses to this, but we are also curious about if anyone has purchased or tested an Elsa Cryo transfer holder and their opinion about it - particularly, if folks have been able to get high resolution data with it.

thanks
Liz Montabana
Donner Labs, LBNL

On Wed, Nov 29, 2017 at 4:54 AM, Anastasia Gazi <anadegaz at gmail.com<mailto:anadegaz at gmail.com>> wrote:
Hello all,

We are considering the purchase of an Elsa Cryo transfer holder Model 698 from Gatan. It is looking great on paper with its centrosymmetric design, extended hold time to 8h and everything but I am wondering if someone has already experience with it. Either with the standard profile holder or with the ultra-low one.

Thank you for your help,
Anastasia

==================================================
Anastasia D. Gazi, PhD.
Ultrastructural BioImaging unit,
Départment de Biologie cellulaire et infection,
Institut Pasteur
28, rue du Dr. Roux
75015 PARIS
FRANCE
agazi at pasteur.fr<mailto:agazi at pasteur.fr>
==================================================

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