[3dem] How to make grids positively charged during or after glow discharge.

[Sacha De Carlo]

You would need to read the old classics by people trying to look at dna in
negative staining: look for old protocols using pentylamine (if I find
them, I will share them with you) but I’m sure there are some review papers
too.

Best
Sacha


Sent from my mobile device
-----------------------------
Sacha De Carlo, Ph.D.
Business Development Manager EM
Dectris.com

On 3 Jul 2019, at 20:06, Ross, Bradford <bradford.ross at botany.ubc.ca> wrote:

Hello Everyone,


I'm currently trying to image some lipid nanoparticles which have a
positive charge on their outer surface. Of course, since normal glow
discharge treatment typically yields a negative charge on the grids, the
particles are all sticking to the lacey carbon support instead of remaining
suspended in the vitreous ice layer.


I have heard that Magnesium Acetate can be used to switch the charge from
negative to positive after glow discharging, but can't seem to find any
protocols for exactly how to treat the grids with it.


Does anyone have a protocol for the use of Magnesium Acetate, or any other
suggestions on how to flip the surface charge of the grids to positive? Or
other creative solutions I'm not thinking of?


Cheers,
Bradford Ross

Electron Microscopy Technician
BioImaging Facility
University of British Columbia
Cunningham Building Rm. 64
2146 East Mall
Vancouver, B.C.
V6T 1Z4

phone 604-822-6996

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