[3dem] How to make grids positively charged during or after glow discharge.

[SCHMUTZ Marc (ICS)]

You mean that paper Sacha ? 


A new preparation method for dark-field electron microscopy of biomacromolecules [ https://www.sciencedirect.com/science/article/pii/S002253207180148X#aep-article-footnote-id1 | 1 ] [ https://www.sciencedirect.com/science/article/pii/S002253207180148X#aep-article-footnote-id2 | 2 ] [ https://www.sciencedirect.com/science/article/pii/S002253207180148X#aep-article-footnote-id3 | 3 ] 
Author links open overlay panel [ https://www.sciencedirect.com/science/article/pii/S002253207180148X#! | JacquesDubochet4 ] [ https://www.sciencedirect.com/science/article/pii/S002253207180148X#! | MichelDucommun4 ] [ https://www.sciencedirect.com/science/article/pii/S002253207180148X#! | MaxZollinger ] [ https://www.sciencedirect.com/science/article/pii/S002253207180148X#! | EdouardKellenberger4 ] 

[ https://www.sciencedirect.com/science/journal/00225320 | Journal of Ultrastructure Research ] 
[ https://www.sciencedirect.com/science/journal/00225320/35/1 | Volume 35, Issues 1–2 ] , April 1971, Pages 147-167 

regards 


Marc 

De: "Sacha De Carlo" <sacha.decarlo at dectris.com> 
À: "Ross, Bradford" <bradford.ross at botany.ubc.ca> 
Cc: 3dem at ncmir.ucsd.edu 
Envoyé: Mercredi 3 Juillet 2019 22:09:02 
Objet: Re: [3dem] How to make grids positively charged during or after glow discharge. 

You would need to read the old classics by people trying to look at dna in negative staining: look for old protocols using pentylamine (if I find them, I will share them with you) but I’m sure there are some review papers too. 
Best 
Sacha 


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Sacha De Carlo, Ph.D. 
Business Development Manager EM 
[ http://dectris.com/ | Dectris.com ] 

On 3 Jul 2019, at 20:06, Ross, Bradford < [ mailto:bradford.ross at botany.ubc.ca | bradford.ross at botany.ubc.ca ] > wrote: 






Hello Everyone, 




I'm currently trying to image some lipid nanoparticles which have a positive charge on their outer surface. Of course, since normal glow discharge treatment typically yields a negative charge on the grids, the particles are all sticking to the lacey carbon support instead of remaining suspended in the vitreous ice layer. 




I have heard that Magnesium Acetate can be used to switch the charge from negative to positive after glow discharging, but can't seem to find any protocols for exactly how to treat the grids with it. 





Does anyone have a protocol for the use of Magnesium Acetate, or any other suggestions on how to flip the surface charge of the grids to positive? Or other creative solutions I'm not thinking of? 





Cheers, 
Bradford Ross 

Electron Microscopy Technician 
BioImaging Facility 
University of British Columbia 
Cunningham Building Rm. 64 
2146 East Mall 
Vancouver, B.C. 
V6T 1Z4 

phone 604-822-6996 




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