[3dem] How to make grids positively charged during or after glow discharge.

[Chen Sun]

Hi Bradford,

In our lab, we have been using poly-L-Lysine as a positive substrate and it has yielded good results consistently with a clear background.


Chen
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Ross, Bradford <bradford.ross at botany.ubc.ca>
Sent: Wednesday, July 3, 2019 14:06
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] How to make grids positively charged during or after glow discharge.


Hello Everyone,


I'm currently trying to image some lipid nanoparticles which have a positive charge on their outer surface. Of course, since normal glow discharge treatment typically yields a negative charge on the grids, the particles are all sticking to the lacey carbon support instead of remaining suspended in the vitreous ice layer.


I have heard that Magnesium Acetate can be used to switch the charge from negative to positive after glow discharging, but can't seem to find any protocols for exactly how to treat the grids with it.


Does anyone have a protocol for the use of Magnesium Acetate, or any other suggestions on how to flip the surface charge of the grids to positive? Or other creative solutions I'm not thinking of?


Cheers,

Bradford Ross

Electron Microscopy Technician
BioImaging Facility
University of British Columbia
Cunningham Building Rm. 64
2146 East Mall
Vancouver, B.C.
V6T 1Z4

phone 604-822-6996

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